白介素10的基因多态性与尖锐湿疣的相关性研究

目的探讨白介素10（IL-10）基因启动子-1082位点基因多态性与尖锐湿疣的相关性。方法采用焦磷酸测序法（Pyrosequencing）检测30例尖锐湿疣患者（观察组）和50例健康体检者（对照组）IL-10基因启动子-1082G/A位点基因型和等位基因频率;同时采用双抗体夹心ELISA法测定对照组和观察组的血清IL-10水平。结果观察组血清IL-10水平显著高于对照组（P〈0.01）。观察组IL-10基因启动子-1082 G/A位点GG基因型分布频率和G等位基因频率高于对照组（P〈0.01）。在观察组中表达GG基因型患者的血清IL-10水平显著高于表达其它基因型患者的血清IL-10水平（P〈0.05）。结论 IL-10基因多态性与尖锐湿疣易感性可能相关,IL-10基因启动子-1082 G/A位点GG基因型携带者对尖锐湿疣的易感性高。

Study on the correlation between the polymorphisms of gene promoter-1082G/A in interleukin 10 and condyloma acuminatum

Objective:To investigate the correlation between the polymorphisms of gene promoter-1082G/A in interleukin 10(IL-10) and condyloma acuminatum.Methods: Pyrosequencing was used to detect the single nucleotide polymorphisms(SNP) of gene promoter-1082G/A in IL-10 of 30 patients with condyloma acuminatum(observing group) and 50 normal controls(control group).ELISA was carried out to detect the serum level of IL-10 of observing group and control group.Results: The IL-10 levels of observing group were higher than that of control group(P<0.05).Frequencies of GG genotype and allele G at position-1082G/A of observing group were significantly higher than that of control group(P<0.01).In the observing group,IL-10 levels of patients expressing GG were higher than that of patients non-expressing GG(P<0.05).Conclusions: The polymorphisms of IL-10 gene may be correlated with condyloma acuminatum.People who carry genotype GG of gene promoter-1082G/A in IL-10 may have susceptibility to condyloma acuminatum.