健脾解毒化瘀方对大鼠实验性溃疡性结肠炎细胞因子的影响

目的]探讨以益气健脾、清热解毒、活血化瘀为原则组成方剂对溃疡性结肠炎(UC)大鼠细胞因子的影响,探讨其治疗UC的机制.方法]除15只大鼠作为正常组外,其他大鼠采用TNBS复制UC模型后随机分成模型组、对照组、柳氮磺胺吡啶(SASP)组、中药组,肉眼及镜下观察各组大鼠的组织学损伤,并检测血清中细胞因子的含量.结果]中药组、SASP组在减轻肉眼及镜下的组织学损伤方面和改善细胞因子方面较模型组、对照组均具有明显效果(P＜0.05)；中药组较SASP组疗效更明显(P＜0.05).结论]以益气健脾、清热解毒、活血化瘀为原则组成方剂对UC治疗有效,其机制可能是提高抗炎因子的含量,降低炎症因子的含量,维持细胞因子之间的平衡,进而调节免疫炎症反应.

Experimental study of the method of strengthening the spleen and supplementing qi,clearing away heat and toxicant,resolving blood-stasis and unblocking collaterals on the cytokines in experimental rats with ulcerative colitis

Objective]To investigate the effects of Chinese formula of strengthening the spleen and supplementing qi,clearing away heat and toxicant,resolving blood-stasis and unblocking collaterals on cytokines of ulcerative colitis(UC)rat,and to explore the mechanism of its treatment of UC. Methods]Except 15 rats as a normal group,other UC rat model by TNBS were randomized into model group,control group,liu nitrogen sulfa-drugs pyridine(SASP) group,traditional Chinese medicine group.Histologic damage and cytokines in serum were detected. Results]Traditional Chinese medicine group and the SASP group had better effect in improving cells damage and the nistologic factor than the control group(P<0.05);curative effect of traditional Chinese medicine group was better than SASP group(P<0.05). Conclusion]The balance of cytokines plays an important role in the pathogenesis of UC.The method of traditional Chinese treatment is effective,which may improve the anti-inflammatory factor content,reduce levels of inflammatory cytokines to maintain the balance among cytokines,thereby regulating the immune and inflammatory responses.