银杏叶提取物抗鼠脑缺血缺氧的药理学机制研究

目的研究新生鼠缺血缺氧性脑损伤后(HIBD)脑组织神经元特异性烯醇化酶(NSE)、S-100蛋白(S-100)mRNA表达以及银杏叶提取物(GbE)对NES、S-100mRNA表达的影响,以探讨GbE抗脑缺血缺氧的药理作用机制。方法90只7日龄SD大鼠随机分成3组,建立HBID模型后,用RT-PCR技术观察脑组织NES、S-100mRNA的动态变化及GbE对其表达的影响。结果脑组织NESmRNA的表达在HIBD后24h达高峰,脑组织S-100mRNA的表达在HIBD后48h达高峰,高于假手术对照组(P<0.01)。以后逐渐下降,至96h仍未降至正常。腹腔注射GbE24、48、72h能增加脑组织NES、S-100mRNA表达的量(P<0.01)。结论GbE通过增加脑组织NES、S-100mRNA的表达改变能量代谢及细胞内Ca2+浓度从而抗脑缺血缺氧。

NES and S-100mRNA level change in newborn rat brain with hypoxic-ischemic brain damage, and the effects of ginkgo biloba extract on their expressions

Objecitve To study the effects of ginkgo biloba extract(GbE)on expressions of neuron-specific enolase(NSE)and S-100 protein(S-100)mRNA in newborn rat brain after hypoxic-ischemic brain damage(HIBD)and the mechanism of GbE against HIBD.Methods The dynamic changes of expressions of NSE S-100mRNA in brain tissue and effects of GbE were studied using 7d-old SD rat hypoxic-ischemic brain damage model and RT-PCR techniques.Results The peak expressions of mRNA for NSE and S-100 occurred at 24 and 48 hours after HIBD respectively,and their expressions decresed gradually.The expressions of mRNA for NSE S-100 at 24,48,72 hours were increased after the treatment with GbE(P<0.01).Conclusion GbE can enhance the expressions of mRNA for NSE and S-100 in brain tissue.This may be one of the mechanisms of GbE against HIBD.