| 真核表达hCAP-18/LL-37成熟肽对树突状细胞表面分子表达的影响 |
| |
| 引用本文： | 袁红艳,王凤丽,许娜,常雅萍.真核表达hCAP-18/LL-37成熟肽对树突状细胞表面分子表达的影响[J].吉林大学学报(医学版),2009,35(3):482-486. |
| |
| 作者姓名： | 袁红艳 王凤丽 许娜 常雅萍 |
| |
| 作者单位： | 1．吉林大学基础医学院免疫学教研室,吉林 长春130021；2．吉林医药学院,吉林 吉林 132013 |
| |
| 基金项目： | 吉林省科技厅科技发展计划 |
| |
| 摘 要： | 目的：研究真核表达的人源阳离子抗菌肽-18(hCAP-18)成熟肽LL-37对树突状细胞(DCs)表面分子表达的影响。方法：通过基因克隆方法构建hCAP-18成熟肽LL-37真核表达载体pcDNA4/Myc-His-LL-37，转染人胚肾HEK293细胞株，转染48 h后收获培养上清，与体外以rh-GM-CSF、rh-IL-4联合培养定向诱导分化的不成熟DCs共培养48 h，收获DCs；流式细胞仪检测DCs表面分子CD40及HLA-DR的表达。结果：成功构建成熟肽真核表达载体pcDNA4/Myc-His-LL-37，并实现在其真核细胞HEK293中的表达；流式细胞仪结果显示，pDNA4/Myc-His-LL-37基因转染HEK293细胞培养上清刺激组DCs中CD40及HLA-DR的表达阳性率与对照组比较均明显升高(P<0.05)。结论：构建了真核表达的hCAP-18成熟肽LL-37，其可上调DCs表面分子CD40及HLA-DR的表达，促进DCs功能的成熟。
|
| 关 键 词： | hCAP-18/LL-37 成熟肽 树突状细胞 表面分子 |
| 收稿时间： | 2008-10-29 |
Effects of eukaryotic expressive mature peptide of hCAP-18/LL-37 on expressions of membrane molecules on dendritic cells |
| |
| YUAN Hong-yan,WANG Feng-li,XU Na,CHANG Ya-ping.Effects of eukaryotic expressive mature peptide of hCAP-18/LL-37 on expressions of membrane molecules on dendritic cells[J].Journal of Jilin University: Med Ed,2009,35(3):482-486. |
| |
| Authors: | YUAN Hong-yan WANG Feng-li XU Na CHANG Ya-ping |
| |
| Institution: | 1. Department of Immunology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China;2.Jilin Medical College,Jilin 132013,China |
| |
| Abstract: | Objective To study the effects of eukaryotic expressive mature peptide LL-37 of human cationic antimicrobial peptide(hCAP-18) on the expressions of membrane molecules on dendritic cells(DCs).Methods By gene cloning,the eukaryotic expressive plasmid pcDNA4/Myc-His-LL-37 for the mature peptide LL-37 of hCAP-18 was constructed.Then they were transfected into HEK293 cell lines.After the cell lines were cultivated for 48 h,the supernatant was collected.Then the DCs from peripheral blood mononuclear cells(PBMCs) ... |
| |
| Keywords: | hCAP-18/LL-37 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《吉林大学学报(医学版)》浏览原始摘要信息 |
| 点击此处可从《吉林大学学报(医学版)》下载免费的PDF全文 |
