中药黄蜀葵花化学成分的分离与鉴定（III）

目的 研究中药黄蜀葵（Abelmoschus manihot (L.）Medic)花的化学成分。方法 采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱以及HPLC等手段进行分离纯化,并通过理化性质与光谱分析方法鉴定了化合物的结构。结果 从黄蜀葵花体积分数为95%的乙醇提取物中分离鉴定了9个化合物,分别为棉皮素 8-O-β-D-葡萄糖醛酸苷（gossypetin 8-O-β-D-glucuronide,1）、棉皮素 3-O-β-葡萄糖-8-O-β-葡萄糖醛酸（gossypetin 3-O-β-glucopyranoside-8-O-β-glucuronopyranoside,2）、棉皮素 3'-O-β-D-葡萄糖苷（gossypetin 3'-O-β-D-glucopyranoside,3）、tiliroside（4）、山奈酚3-O-[(3"-O-乙酰基-6"-O-(E)-对羟基桂皮酰基)]-β-D-葡萄糖苷（kaempferol 3-O-[3"-O-acetyl -6"-O-(E)-p-coumaroyl)]-β-D-glucopyranoside,5）、槲皮素 3-O-[β-D-木糖基(1→2)-a-L-鼠李糖基(1→6)]-β-D-半乳糖苷（quercetin 3-O-[β-D-xylopyranosyl(1→2)-a-L-rhamnopyranosyl(1→6)-β-D-galactopyranoside,6）、4-羟基苯甲酸 β-D-葡萄糖酯（4-hydroxybenzoic acid β-D-glucose ester,7）、原儿茶酸（protocatechuic acid,8）、原儿茶酸 3-O-β-D-葡萄糖苷（protocatecheuic acid 3-O-β-D-glucoside,9)。结论 其中化合物2、4-9为首次从秋葵属中分离得到,并首次报道了化合物5、6、9在DMSO-d₆中的光谱数据。

Isolation and identification of chemical constituents from the flowers of Abelmoschus manihot (L.) Medic (III)

Objective To study the chemical constituents from the flowers of Abelmoschus manihot (L.) Medic. Method The compounds were isolated by chromatographies such as silica gel, ODS, Sephadex LH-20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis. Results Nine compounds were isolated. The structures of them were identified as gossypetin 8-O-β-D-glucuronide (1), gossypetin 3-O-β-glucopyranoside-8-O-β-glucuronopyranoside (2), gossypetin 3'-O-β-glucopyranoside (3), tiliroside (4), kaempferol 3-O-[3"-O-acetyl -6"-O-(E)-p-coumaroyl)]-β-D-glucopyranoside (5), quercetin 3-O-[β-D-xylopyranosyl(1→2)-a-L-rhamnopyranosyl(1→6)]-β-D-galactopyranoside (6), 4-hydroxybenzoic acid β-D-glucose ester (7), protocatechuic acid (8), protocatecheuic acid-3-O-β-glucoside (9), respectively. Conclusion Seven compounds 2, 4-9 are obtained from Abelmoschus genus for the first time, and the spectral data in DMSO-d₆ solution of 5, 6 and 9 are reported firstly.