植物基因组DNA提取方法学评析与验证

分子生物学实验多以提取研究对象的基因组DNA作为研究的起点,由于材料的来源、部位、形态等外在性质的不同,以及化学成分、组织结构等内在特点的差异,常会需要在提取基因组DNA时选择不同的方法,或作一些特殊的处理,如有的植物组织含有较多的多糖及酚、酯、萜等次生代谢产物,从而会影响到所提DNA的纯度及随后的实验分析。因而选择一种简单、快速而高效提取基因组DNA的方法常常成为实验中关键的一步。近几年来已有不少关于通用提取方法的报道,另外一些专属性的方法也有了较大进展。本文拟对这些方法加以总结、比较,以期对它们各自的特点作一剖析,并加以实验改进验证,以期能够在今后的实验中更好地应用。

Evaluation and Authentication of Methodology on Genomic DNA Isolation of plant

A simple, fast and effective method to isolate Genomic DNA from different materials is often the important step of the molecular biological experiments. In order to obtain high quality of genomic DNA to continue kinds of downstream applications,it is necessary to choose an appropriate extraction method according to different characters such as the origin, macroscopical, microscopical,physical and chemical markers of kinds of research objects, There have been many papers introducing universal isolation method of genomic DNA in recent years. We should summarize, compare, validate and modify these different methods in the present paper, so as to apply these methods better later.