中国HIV-1感染者病毒特异性细胞毒性T细胞定量研究

目的对8例HIV长期感染不进展(LTNP)及病程进展缓慢(SP)患者的病毒特异性细胞毒性T细胞(CTL)特征进行研究。方法设立队列研究,从中随机选出8例患者(4例LTNPs,4例SP)。通过采用重叠肽技术组建HIV-1B亚型全序列肽段,组建成三维肽库进行ELISPOT分析8例患者的HIV-1特异性细胞免疫反应。结果在大多数患者中存在较强的T细胞反应,特别对HIV-1病毒的pol、gag、nef蛋白的免疫反应比其他蛋白强。结论病毒特异性CTL免疫反应在HIV-1 LTNPs有较强并且很广泛的反应,这对于有效抑制病毒在人体内的生存有可能起到很大的作用,很可能是这些LTNPs及SP病程进展缓慢的主要原因之一。

High Frequency CTL Responses in HIV-1-Infected Chinese Plasma Donors

Objective To study the magnitude and breadth of HIV specific T cell responses in 8 HIV infected long-term nonprogressors(LTNPs) and slow progressors(SP) who received contaminated blood.Methods PBMCs were tested in an Elispot assay against a matrix of peptides(15 mers overlapping by 11 amino acids) representing the full proteome of HIV clade B virus.The peptides were presented as 3 dimensional matrixes such that each peptide appears in 3 unique wells enabling the epitope to be identified.Results We found broad T cell responses in most donors,although in one individual,a strong response against only gag protein was observed and T cells responses to pol,gag and nef were more dominate than the others.Conclusion High frequency and broad HIV specific T cells detected in Chinese HIV infected LTNP and SP individuals are very likely associated with the control of the disease progression in those individuals.