转粒细胞巨噬细胞集落刺激因子基因瘤苗治疗T细胞淋巴瘤的实验研究

目的:探讨转粒细胞巨噬细胞集落刺激因子(granulocyte-macrophge colong stimulating factor,GM-CSF)基因瘤苗治疗小鼠T淋巴细胞瘤的方法。方法:将小鼠GM-CSF真核表达质粒用电穿孔法导入小鼠淋巴瘤细胞系RMA,有限稀释法制备单个细胞克隆,经RT-PCR、骨髓祖细胞增殖实验和集落形成实验筛选相对高表达GM-CSF的RMA克隆,该克隆细胞经丝裂霉素灭活后免疫小鼠以诱导其产生抵抗RMA肿瘤细胞再攻击的能力。结果:获得了高表达GM-CSF的RMA克隆,将其用丝裂霉素灭活后免疫小鼠使小鼠使小鼠产生了抗肿瘤免疫保护力。结论:转GM-CSF基因瘤苗可作为有效的抗肿瘤瘤苗。

Experimental study of T cell lymphoma immunotherapy by tumor cells transfected with GM-CSF gene

Objective : To study the method of leukemia treatment with tumor cells transfected with GM-CSF gene. Methods : GM-CSF eukaryotic expressing plasmid was transfected into RMA cells by electro-poration . After screening by G418 and cloning by limiting dilution,a relative high GM-CSF expressing cell clone was selected by RT-PCR. Hematopoietic progenitor cell proliferative assay and hamatopoietic progenitor cell colony formation assay. The cells of this clone were inactivated by mitomycin-C and vaccinated mice to induce anti-tumor immune reaction. Results : A GM-CSF high expressing cell line RMA-GM was selected, which can induce mice obtain anti-tumor immune protective ability. Conclusion : tumor cells transfected with GM-CSF gene are used as a effective anti-tumor vaccine.