| 风疹病毒山东地方株的分离与鉴定 |
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| 引用本文： | 王同展,宋艳艳,王常银,徐爱强,宋立志,许青,肖作奎,张丽,李漫时. 风疹病毒山东地方株的分离与鉴定[J]. 中国公共卫生, 2004, 20(6): 693-694 |
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| 作者姓名： | 王同展 宋艳艳 王常银 徐爱强 宋立志 许青 肖作奎 张丽 李漫时 |
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| 作者单位： | 1. 山东省疾病预防控制中心,济南,250014
2. 山东大学公共卫生学院 |
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| 摘 要： | 目的 了解山东省风疹病毒流行情况，进行风疹野病毒山东地方株的分离、鉴定。方法 2001～2002年，从我省的济宁、淄博、济南、德州市风疹爆发流行中，采集53份具有风疹典型症状患者的咽拭子，经Vero，RK-13，BHK-21，MK2细胞分离培养，选出有明显的细胞病变株。结果 在Vero细胞上对HSV-1病毒攻击呈干扰现象；风疹免疫荧光阳性者；共分离出9株风疹病毒山东地方野毒株(SDRV)，代码为SDRV1、2、3、4、5、6、7、8、9。结论 细胞用于风疹病毒的分离培养，免疫荧光实验可作为风疹病毒鉴定、诊断的重要方法。
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| 关 键 词： | 风疹病毒 免疫荧光试验 Veto细胞 |
| 文章编号： | 1001-0580(2004)06-0693-02 |
| 修稿时间： | 2003-09-01 |
Isolation and identification of rubella virus strains from Shandong province |
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| WANG Tong-zhan,SONG Yan-yan,WANG Chang-yin,et al.. Isolation and identification of rubella virus strains from Shandong province[J]. Chinese Journal of Public Health, 2004, 20(6): 693-694 |
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| Authors: | WANG Tong-zhan SONG Yan-yan WANG Chang-yin et al. |
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| Affiliation: | WANG Tong-zhan,SONG Yan-yan,WANG Chang-yin,et al.Shandong Center for Disease Control and Prevention |
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| Abstract: | Objective For isolation and identification of rubella virus wild strain from Shandong Province.Methods 9 wild strains of Shandong district of rubella virus were isolated from 53 throat swabs.These virus cause marked cytopathic effect (CPE) in Vero,RK-13,BHK-21,MK2 cell lines.Results 9 SDRV strains were isolated.SDRV infected Vero cell showed the interference with the invasion of HSV-1,Immunofluorescence assay were used to identify the viruses,neutralization tests were used for confirmation for newly rubella virus isolations.Conclusion Immuofluorescene can be the important method for rubella virus isolation and identification. |
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| Keywords: | rubella virus immuno-fluorescence assay vero cell |
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