内耳免疫反应中内耳血管细胞粘附分子-1和α4-整合素的表达

目的 探讨血管细胞粘附分子-1(vascular cell adhesion molecule-1，VCAM-1)和α4-整合素(α4-Integrin)在内耳免疫反应中的作用。方法 采用钥孔戚血蓝蛋白激发已两次全身致敏的大鼠内耳，诱发其内耳免疫反应，制造迷路炎模型，然后通过免疫组织化学技术，检测内耳VCAM-1和α4-Intergrin分别在内耳中的表达。结果 内耳激发后24小时，螺旋轴静脉(spiral modiolar vein，SMVs)及其回流小静脉(collecting venules,CVs)可见有VCAM-1表达，48小时达最高峰，维持此水平1周，然后下降。α4-Intergrin在致敏后24小时即可表达于浸润于SMV8和CVs及蜗神经周围的白细胞，铝小时达高峰，然后迅速下降。结论 内耳免疫反应时VCAM-1、α4-Intergrin在炎性细胞从循环系统进入内耳的过程中发挥着重要作用。

Expressi of Vascular Cell Adhesion Molecule-1 and α4-Integrin During Inner Ear Immune Response

Objective　To investigate the expression of vascular cell adhesion molecule-1(VCAM-1) on the spiral modiolar vein(SMVs) with its collecting venules(CVs), as well as the expression of α4-integrin on leukocytes, during inner ear inflammation induced by immunity.Methods　Labyrinthitis was induced in rats by inoculation of keyhole limpet hemocyanin(KLH) into the scala tympani of animals that had been secondly systemically sensitized to it. Expression of VCAM-1 and α4-integrin was examined with polycolonal or monoclonal antibodies to rat VCAM-1 and α4-integrin by immunohistochemistry.Results　VCAM-1 was found on the endothelium(EC) of SMVs and CVs in 24 hours postchallenge, reaching a maximum by day 2, and maintaining the high lever till one week postchallenge, then fading away gradually. By two week, it showed that almost no significant staining with anti-VCAM-1. α4-integrin was found on the surface of leukocytes that infiltrated in SMVs、CVs、cochlear nerve and perilymph, mainly monocytes and lymphocytes, in 24 hours postchallenge, reaching a maximum by day 2, then fading away quickly. After one week postchallenge, significant expression of α4-integrin was observed. Conclusion　The study further elucidated the role of adhesion molecules in extravasation of inflammation cells from blood vessels during an inner ear immune response.