Study on the effects of losartan on cardiomyocyte apoptosis and gene expression after ischemia and reperfusion in vivo in rats |
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Authors: | Zhang Dongqing Yang Liming Liu Zhengxiang Mi Shizan |
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Institution: | Department of Cardiology, Tongji Hospital, Tongji Medical University, Wuhan 430030 |
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Abstract: | Summary In order to study the effects of losartan on cardiomyocyte apoptosis following ischemia (0.5 h) and reperfusion (48 h) in
vivo and bcl-2 and bax gene expression, TUNEL staining method, immunohistochemistry and in situ hybridization histochemistry
(ISHH) were used to monitor the apoptotic cells, mRNA and protein of gene expression, respectively. Image processing system
was used to quantitively dispose the positive metric substance of both immunohistochemistry and ISHH through the average optical
density (OD) value. The number of the apoptotic cells were 38 ±9 (control group), 0–1 (sham operation group) and 9±4 (losartan-treated
group) in each visual field respectively with the difference among the groups being significant (P < 0.001). OD values of bcl-2 (ISHH) were 0.07425 ± 0. 02029 (control group), 0. 05961 ± 0.009932 (sham operation group) and
0.07619±0. 01445 (losartan-treated group) respectively, while OD values of bcl-2 (immunohistochemistry) were 0.1374 ± 0.01367
(control group), 0. 08510±0. 01862 (sham operation group) and 0.1252±0.02064 (losartan-treated group), bcl-2 gene expression
was increased significantly in the control group and losartan-treated group as compared with sham operation group (P < 0.05). OD value of bax (immunohistochemistry) was 09727±0.02230 (control group), 0.06182 ± 0.01430 (sham operation group)
and 0.06213 ± 0.01420 (losartan-treated group). bax gene expression was decreased very significantly in losartan-treated group
and sham operation group as compared with control group (P < 0.001). Bcl-2/ bax ratio was 1.413 (control group), 1.376 (sham operation group) and 2.016 (losartan-treated group) respectively.
The results indicated that losartan might inhibit cardiomyocyte apoptosis following ischemia and reperfusion. The mechanism
might be that bax gene expression was inhibited to increase bcl-2/bax ratio. |
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Keywords: | losartan ischemia/reperfusion cardiomyocyte apoptosis gene modulation |
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