| Effects of Cigarette Smoke Extract on E-cadherin Expression in Cultured Airway Epithelial Cells |
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| 作者姓名： | 王曦 吴人亮 郝天玲 陈芳 |
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| 作者单位： | WANG Xi，WU Renting，HAO Tianling，CHEN Fang (Department of Pathology，School of Basic Medical Sciences，Tongji Medical University，Wuhan 430030) (Pulmonary Disease Laboratory，Ministry of Health，Tong n Medical University，Wuhan 430030) |
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| 基金项目： | This project was supported by a grant from National Natural Science Foundation of China !(39570288). |
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| 摘 要： | E--cadherin (ECD) is an important cell--cell adhesion molecule which maintains the polarity and..integrity of respiratory eplthelial cellsLlj. Smokingmay cause chronic bronchitis and emphysema, thenchronic ocr pulmonale']. The early pathologicalchange of chronic bronchitis is characterized by thedamage to cilla--mucus transport system, degeneration, necrosis and shedding of the epithelial cells.In this study, we cultured porcine airway epithelialcells (AECs ) to investigate whether cigaret…
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| 收稿时间： | 12 September 1999 |
Effects of cigarette smoke extract on E-cadherin expression in cultured airway epithelial cells |
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| Wang Xi,Wu Renling,Hao Tianling,Chen Fang.Effects of Cigarette Smoke Extract on E-cadherin Expression in Cultured Airway Epithelial Cells[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2000,20(1):32-35. |
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| Authors: | Wang Xi Wu Renling Hao Tianling Chen Fang |
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| Institution: | 1. Department of Pathology, School of Basic Medical Sciences, Tongji Medical University, Wuhan 430030
2. Pulmonary Disease Laboratory, Ministry of Health, Tongji Medical University, Wuhan 430030 |
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| Abstract: | Summary: To investigate whether the change of E-cadherin (ECD) expression plays a role in the injury and repair of airway epithelial cells (AEC) caused by smoking, porcine AECs were cultured by using an enzyme-dispersed method. After exposure of the AECs to cigarette smoke extract (CSE), the ECD expression in the cells was detected by using immunocytochemistry and in situ hybridization. The results showed that ECD was distributed on the plasma membrane at the cell junctions of AECs. After exposure to 20 % CSE, the membranous ECD expression was decreased, the cytoplasmic ECD expression was increased (P<0.01) as the exposure time went on. But the content of ECD mRNA in the AECs did not chang. It suggests that the change of ECD ex- pression is regulated at the posttranslational level and plays a role in the injury and repair of AEC caused by smoking. |
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| Keywords: | airway epithelial cell E-cadherin smoke inhalation injury |
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