| In Vitro Expression of Hepatitis C Virus Non-structure 5 Antigen in the HepG2 Cell Line |
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| 作者姓名： | 叶进 曾令兰 杨木兰 罗端德 郭劲松 |
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| 作者单位： | Department of Infectious Disease，Xiehe Hospital，Tongji Medical University，Wuhan 430022; Laboratory of Ultra-Pathology of Experiment Center，Tongji Medical University，Wuhan 430033 |
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| 基金项目： | This project was supported by a grant of the National Edu-cational Committee for return scholars (No. 95-8067) |
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| 摘 要： | ItisofgreatsignificancetoestablishasensitivepropagationsystemofHCVinvitroforstudyingthefeaturesofHCV,pathogenesisofHepatitisC,thetreatmentofanti--virusandstudyofvaccine.Wede-tectedthepositiveandnegativelineofHCVinTlymphcellsofhumaninndroinpreviousstudyLI],andpresentedinthispaPeristhefurtherstudyoninvljroexpressionofHCV.lMATERMisANDMETHODS1'1PositiveInocuIumsofHCVRNATheserumofHCVRNAwith1evelof2X10'copy/mlwasselected,anddefinedbyfluorescentquantificationmethed(Thereagentkitwa…
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In Vitro Expression of Hepatitis C Virus Non-structure 5 Antigen in the HepG2 Cell Line |
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| YE Jin,ZENG Linglan,YANG Mulan,LUO Duande,GUO Jinsong.In Vitro Expression of Hepatitis C Virus Non-structure 5 Antigen in the HepG2 Cell Line[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2000,20(4). |
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| Authors: | YE Jin ZENG Linglan YANG Mulan LUO Duande GUO Jinsong |
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| Institution: | 1. Department of Infectious Disease, X iehe Hospital, Tongji Medical University, Wuhan 430022
2. Labiratirt of Ullra-Pathology of Experiment Center,Tongji Medical University,Wuhan 430033 |
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| Abstract: | To establish a cell line as a model system for HCV infection and propagation in vitro, a human HepG2 cell line was incubated with a HCV RNA positive serum. The sABC immunological techniques and gold-labeled colloid electron microscopy method were employed to examine the viral proteins in those cells. The HCV non-structure 5 antigen was first detected in the HepG2 cells 72 h after incubation. The antigen was continuously observed in the cytoplasm as well on the membrane of the HepG2 cells even after 1. 2, 3 and 4 weeks after incubation. The observation of HCV non-struc- ture 5 antigen continuously expressed in the HepG2 cells strongly indicates that the cells may have been infected by HCV virus. Therefore, the HepG2 cell line may serve as a potential host for estab- lishment of HCV infection and propagation in vitro. |
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| Keywords: | hepatitis C virus infection antigen expression |
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