| Study on Biologic Activity for Membrane of Normal Bone Marrow Cells with Infection of Epidemic Hemorrhagic Fever Virus |
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| 作者姓名： | 张建傲 曾令兰 罗端德 蔡淑清 刘薇 杨渝珍 郭劲松 |
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| 作者单位： | Department of Infectious Disease，Xiehe Hospital，Tongji Medical University，Wuhan 430022 |
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| 摘 要： | Membranelipidfluidity(MLF)hascorrelationtothemultiplicationandma1ignantdegreeofce11s,whichplaysanimPOrtantroleinthepathOgenesisoftheneoplasiacellsescapingfromthegrowthcontrol.Atpresent,noreportonthebio1ogicchangesinMLFofnormalbonemarrowcellswithinfectionofEHFV.ThepurposeofthisstudywastostudythechangesinMLFofnormalbonemarrowce11sanditsrelationtotheinfectionofEHFV.1MATERIALSANDMETHODS1'lCeIlcultureThepuncturewasmadeontheanteriorsuperioriliacspineorposteriorsuperioriliacspineof27c…
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| 收稿时间： | 17 January 2000 |
Study on biologic activity for membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus |
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| Zhang Jian’ao,Zeng Linglan,Luo Duande,Cai Shuqing,Liu Wei,Yany Yuzhen,Guo Jingsong.Study on Biologic Activity for Membrane of Normal Bone Marrow Cells with Infection of Epidemic Hemorrhagic Fever Virus[J].Journal of Zuazhong University of Science and Technology: Medical Edition,2000,20(4):322-323. |
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| Authors: | Zhang Jian’ao Zeng Linglan Luo Duande Cai Shuqing Liu Wei Yany Yuzhen Guo Jingsong |
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| Institution: | Departnent of Infectious Disease, Xiehe Hospital, Tongji Medical University, Wuhan 430022 |
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| Abstract: | Using DPH fluorescence probe, the membrane of normal bone marrow cells with infection of epidemic hemorrhagic fever virus (EHFV) was labeled. The membrane lipid fluidity was obviously decreased from the membrane lipid fluorescence polarization. The membrane lipid fluidity of lympho- cyte, monocyte and neutrophilic granulocyte was dynamically observed. After culturing the cells for 1, 6, 24 and 72 h, it was found that all the membrane lipid fluidity of the infected cells was de- creased obviously with the longer the culturing time, the more obvious it. Compared with the normal control groups, there was a significant difference statistically (P<0. 05-0. 01). It was suggested that the decrease of the membrane lipid fluidity of normal bone marrow cell with infection of EHFV had correlation with the degree of virus invading and cellfunction injury. |
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| Keywords: | epidemic hemorrhagic fever virus bone marrow cell membrane lipid fluidity |
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