Expression of the inducible nitric oxide synthase isoform in chorionic villi in the early spontaneous abortion |
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Authors: | Xia Geqing PhD MD Sun Yongyu |
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Institution: | Department of Obstetrics & Gynecolog y , Xiehe Hospital, Tongji Medical College, Wuhan 430022 |
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Abstract: | Summary To investigate the relationship between inducible nitric oxide synthase (iNOS) and the early spontaneous abortion.,in situ hybridization and immunohistochemistry were used to detect the expression of iNOS in trophoblasts in the early pregnancy
with and without spontaneous abortion (group I and groupI). By light microscopy and computer color magic image analysis system (CMIAS), light density (D) and the positive cell number
per statistic square (N/S)in situ hybridization were used to analyze the positive cell index, while total positive cells (N) and the positive unit (Pu) were
used in immunohistochemistry. Byin situ hybridization, D and N/S in trophoblasts were 0. 35 ± 0. 028, 0. 07 ± 0. 011 respectively in group I and 0. 18 ± 0. 016,0.
015 ± 0. 003 in groupI. In terms of immunohistochemical staining, N and Pu were 0. 058 ± 0. 007, 11. 94 ± 2. 01 in group I and 0. 013 ± 0. 009,
1. 08 ± 0. 35 in groupI in trophoblasts. Significant differences existed between two groups. It is concluded that the higher nitric oxide produced
by the higher expression of iNOS in trophoblasts might play an important role in the early spontaneous abortion. |
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Keywords: | iNOS nitric oxide spontaneous abortion |
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