日本血吸虫重组抗原rSj14-3-3免疫保护作用的初步研究

目的　初步探讨重组信号蛋白 14 3 3及 14 3 3与GST融合蛋白抗日本血吸虫尾蚴感染和抗血吸虫病的免疫保护作用。　方法　用rSj14 3 3和rSj14 3 3 /SjGST免疫BALB/c小鼠 ,日本血吸虫尾蚴经腹部皮肤攻击感染 ,收集实验组与对照组成虫和虫卵 ,计算减虫率和减卵率 ;间接ELISA法测定实验组与对照组小鼠免疫前、后血清中特异性IgG抗体水平的变化 ;显微镜下测量并比较实验组与对照组肝脏切片上单个虫卵肉芽肿大小 ,观察两种重组抗原对小鼠肝脏肉芽肿形成的影响。　结果　上述两种重组抗原在尾蚴攻击感染后的减虫率分别为 3 1.93 %和 3 4.3 9% ;每克肝组织减卵率分别为 5 3 .2 4%和 60 .0 6% ,每对成虫减卵率分别为 3 3 .3 9%和 40 .48% ;免疫前各组血清IgG抗体A值差异无显著性 ,免疫后实验组血清IgG抗体A值明显高于对照组 ;实验组小鼠肝脏虫卵肉芽肿平均直径比对照组分别下降3 5 .2 3 %和 46.13 %。　结论　信号蛋白 14 3 3在抗感染和抗病免疫中具有保护作用 ,复合多价疫苗的免疫保护作用可能优于单价疫苗。

PRIMARY STUDY OF PROTECTIVE IMMUNE OF SCHISTOSOMA JAPONICUM RECOMBINANT ANTIGEN(rSj14-3-3)

Objective To study the immuno-protection of rSj14-3-3 and rSj14-3-3/SjGST against Schistasoma japonicum in mice. Methods The BALB/c mice were immunized 3 times with rSj14-3-3 and rSj14-3-3/SjGST and then challenged with S. japonicum cereariae via abdominal skin after 5 days last immunization. S. japonicum adult worms and eggs were collected after 6 weeks challenged,and the worm reduction rate and egg reduction rate were calculated respectively. By indirect ELISA,the A value of specific IgG antibodies in BALB/c mice before and after immunization were estimated. The two recombinants' effection to the formation of egg granuloma in liver were observed,the average diameter of single egg granulomas was estimated under microscope. Results The worm reduction rates of rSj14-3-3 and rSj14-3-3/GST groups were 31.93% and 34.39% respectively;The egg reduction rates per gram liver tissue were 53.24% and 60.06%,and the egg reduction rates per worm pair were 33.39% and 40.48%. Before immunization,the difference of the A value of specific IgG antibodies in every mouse was not apparent,but it was apparent after immunization. Compared with control group,the average diameter of egg granulomas in liver of immunized mice reducted 35.23% and 46.13 % respectively. Conclusion Signaling protein 14-3-3 has immuno-protection not only in anti-infectious immune but also in anti-schistosomiasis. The immuno-protection of combinant polyvalency vaccine is probably better than that of monovalency vaccine.