Effect of dietary protein status on urea metabolism and hepatic arginase activity of the pig |
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| Affiliation: | 1. Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Animal Nutrition and Human Health Laboratory, School of Life Sciences, Hunan Normal University, Changsha City, Hunan, 410081, China;2. Chinese Academy of Science, Institute of Subtropical Agriculture, Research Center for Healthy Breeding of Livestock and Poultry, Hunan Engineering and Research Center of Animal and Poultry Science and Key Laboratory for Agroecological Processes in Subtropical Region, Scientific Observation and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Changsha City, Hunan, 410125, China;2. Micronutrients, Indianapolis, IN 46231;3. Department of Animal Science, North Carolina State University, Raleigh 27695;2. University of Nebraska Panhandle Research and Extension Center, Scottsbluff 69361;3. Merck Animal Health, Desoto, KS 66018;4. Cattlemens Nutrition Services, Lincoln, NE 68506, |
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| Abstract: | Experiments were conducted with cross-bred pigs to determine the effect of dietary protein concentration on liver arginase activity. In Exp. 1, pigs were fed diets containing 12, 18 or 24% protein. In Exp. 2, pigs were fed diets containing 12 or 24% protein. Pigs used in Exp. 1 were slaughtered after reaching a wt of 50 kg and after reaching 30, 60 and 90 kg wt in Exp. 2. Aliquots of a 50,000 x g liver supernatant were used for determination of arginase activity as well as endogenous activation kinetics. In addition, aliquots of the supernatants were fractionated by gel chromatography to determine the molecular species of swine liver arginase. Hepatic arginase activity was similar in pigs fed diets containing either 12 or 18% protein but greater in pigs fed the 24% protein diet. The data also indicate that greater than 70% of the hypatic cytosolic manganese is associated with arginase and that arginase activation may be regulated by manganese. |
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