光动力疗法治疗鲜红斑痣的基础研究—血啉甲醚与血卟啉衍生物吸收特性的比较

目的　观察新型光敏剂——血啉甲醚(HMME)在鸡冠皮肤组织和血管内皮细胞(EC)的吸收特点。 方法　莱亨鸡12只，分为HMME组和血卟啉衍生物(HpD)组，每组6只。分别静脉注射HMME或HpD10mg/kg，每隔10min取血2.5ml和鸡冠皮肤组织2.5g。培养新生儿脐带EC，培养液中加入HMME或HpD，孵育浓度分别为20、40、60、80和100 μg/ml，孵育时间为即刻、15、30、60、120、180和240min。采用荧光分析法测定光敏剂含量，并绘制EC吸收光敏剂的浓度—含量和时间—含量关系曲线。 结果　静脉注射HMME或HpD后，其血清浓度的峰值出现在注射后10min，以后随时间迅速下降，二者的曲线形态基本一致。HMME在鸡冠组织中的含量于注射后10min显著高于HpD（P＜0.01），以后虽然下降速率较快，但在注射后80min仍显著高于HpD（P＜0.05）。培养EC可迅速吸收HMME和HpD，30min达峰值的89%以上；其吸收量与孵育浓度呈线性关系，对HMME的吸收量显著高于HpD（P＜0.01）。 结论　HMME较HpD更容易被皮肤微血管EC摄取，这将有利于鲜红斑痣的治疗。

Absorptive Characteristics of HMME and HpD in Chicken Comb Skin and Vascular Endothelial Cells

Objective Port wine stain (PWS) is a congenital vascular malformation of capillaries in the superficial layers of the skin. A new photosensitizer, Hematoporphyrin monomethyl ether(HMME), has been used for photodynamic therapy of PWS. But the absorptive characteristics of HMME in vascular endothelial cell (EC) are still unknown. The purpose of this study is to provide a basis for photodynamic therapy using HMME. Methods 12 Leghorn chickens were divided into 2 groups and HMME or HpD were injected intravenously at a dose of 10 mg/kg. The blood and comb skin sample were taken at 10 min intervals after injection. Human vascular endothelial cells (EC) were cultured and HMME or HpD were added into culture medium in different concentration. Then, EC were incubated for different time. The contents of HMME or HpD in blood, comb skin and EC samples were measured by using spectrofluorophotometer.Results The blood clearance of HMME and HpD were rapid and similar. But the content of HMME in comb skin was significantly higher than that of HpD (P<0 01), especially in 10, 20, 30 min after injection. HMME and HpD can be absorbed rapidly by EC and 89% of the uptake peak value were reached at 30 min after incubation. The uptake quantities of HMME and HpD were positively related with their incubating concentrations and the mean value of HMME absorbed by EC was 155% higher than that of HpD (P<0 01). Conclusions HMME can be absorbed by EC of vascular network more rapidly and easely than HpD. This is advantageous to photodynamic therapy of PWS.