用细胞膜色谱模型筛选长春七抑制HeLa细胞增殖的活性成分

 目的筛选药用植物长春七中具有细胞毒性作用的活性成分。方法用细胞膜色谱模型,筛选条件:红细胞膜色谱柱(50mm×2mm),流动相为三蒸水,柱温37℃,检测波长244nm,流速0.2 mL·min^-1,以阿霉素为对照进行筛选;对筛选得到的活性成分用紫外、红外和质谱方法测定结构,并与文献数据比较确定为蛇床子素;并用MTT法测定了其对HeLa细胞增殖的抑制作用。结果长春七所含蛇床子素在红细胞膜色谱模型系统中,具有类似于阿霉素的保留特性;蛇床子素可抑制HeLa细胞增殖,且随着浓度增加而抑制作用增强,最大抑制率达76.6%,半数抑制浓度为10.0～11.0μg·mL^-1。结论红细胞膜色谱模型可用于筛选药用植物中的细胞毒性成分,长春七所含蛇床子素能够作用于红细胞膜及膜蛋白表面,并对HeLa细胞增殖有较强的抑制作用。

Screening active component inhibiting HeLa cell proliferation in Libanotis buethorimensis by using the model of cell membrane chromatography

OBJECTIVE To screen the active component with cytotoxicity from Libanotis buethorimensis.METHODS The model of cell membrane chromatography (CMC) was used in this study. The chromatographic conditions consisted of the red-blood CMC column (50 mm × 2 mm) with temperature 37℃, The detection wavelength was at 244 nm. The mobile phase was water with a flow-rate of 0.2 mL·min^-1. Adriblastine was used as control drug in the screen. The chemical construction of active component was determined as osthol with UV, IR, MS methods and compared with literature data. The inhibition of osthol on HeLa cell proliferation was analyzed by the MTT method. RESULTS In CMC system,osthol owned a retention characteristics as same as that of adriblastine. It inhibited HeLa cell proliferation and positively correlate with its concentration.The maximum inhibitory rate was up to 76.6% and the 50% inhibitory concentration (IC_{50) was abtained over the range of 10.0～11.0Τg· mL^-1.CONCLUSION The CMC model can be used for screening the cytotoxicity components in medicinal plant.And osthol in Libanotis buethorimensis has a potential inhibition on HeLa cell proliferation.}