高渗氯化钠溶液复苏对失血性休克大鼠T淋巴细胞亚群的早期影响

目的 探讨高渗氯化钠溶液(HS)复苏对失血性休克大鼠T淋巴细胞亚群的早期影响及其意义.方法 将18只SD大鼠制作成重度失血性休克模型,随机分为假手术组(Sham组)、高渗氯化钠溶液复苏组(HS组)和等渗盐水复苏组(NS组),每组6只,采用双抗体标记流式细胞分析技术测定休克前及复苏/急救后各组大鼠的外周血CD4+、CD8+的百分率及二者比值CD4+/CD8+.结果 在失血性休克/复苏/急救后的早期阶段,HS组和NS组大鼠的外周血CD4+细胞亚群表达均显著升高(P＜0.05),HS组大鼠的外周血CD8+细胞亚群表达也有所升高,而NS组大鼠的外周血CD8+细胞亚群表达则无明显改变,从而导致NS组大鼠的外周血CD4+/CD8+比值较Sham组和HS组明显增高,差异具有统计学意义(P＜0.05).结论 在重度失血性休克大鼠模型中,与NS复苏相比较,HS复苏能明显减轻复苏后早期的免疫炎症调节功能紊乱,有助于维持T细胞的辅助-抑制免疫炎症调节网络的平衡.

Early effect of hypertonic saline resuscitation on T-lymphocyte subsets in rats with hemorrhagic shock

Objective To investigate the early effect of hypertonic saline resuscitation on T-lymphocyte subsets in rats with hemorrhagic shock.Methods A model of severe hemorrhagic shock was established in 18 SD rats.The rats were randomly divided into Sham group,HS group (hypertonic saline resuscitation group) and NS group (normal saline resuscitation group).Each group contained 6 rats.The CD4+ and CD8+ subsets of T-lymphocyte in the peripheral blood were detected respectively before shock and after resuscitation by double antibody labelling and flow cytometry.Results In the early stage after hemorrhagic shock,fluid resuscitation and emergency treatment,CD4+ lymphocytes in the HS and NS groups significantly increased(P＜0.05).Small volume resuscitation with HS also induced CD8+ lymphocytes to a certain extent,whereas NS resuscitation showed no effect in this respect.Consequently,compared with Sham and HS groups,CD4+/CD8+ ratio in the peripheral blood of NS group was significantly increased(P＜0.05).Conclusions In this model of rat with severe hemorrhagic shock,small volume resuscitation with HS is more effective than NS in reducing immunologic derangement and promoting a more balanced profile of T-lymphocyte subsets regulating network.