睾丸生殖细胞肿瘤瘤旁原位癌病变组织病理及免疫组化研究

目的观察睾丸原位癌病理形态学改变并探讨胎盘碱性磷酸酶(PLAP)的表达及意义。方法对14例睾丸生殖细胞肿瘤瘤旁原位癌病变组织进行HE染色观察,采用免疫组化方法检测其PLAP表达。结果原位癌病变在曲细精管呈弥漫分布,受累曲细精管变细,基底膜增厚,管内生精细胞消失,仅见癌细胞和Sertoli细胞。癌细胞体积大,核硕大,染色质细,常见居中核仁,胞质透亮,瘤细胞靠近基底膜排列,管内Sertoli细胞正常。免疫组化结果显示,14例睾丸原位癌均表达PLAP,其中12例呈强( ),正常生精细胞及阴性对照无PLAP表达。结论睾丸原位癌呈特征性的病理形态学改变,癌细胞高表达PLAP,表明PLAP是一种特异性强、灵敏度高的肿瘤标记物,它在睾丸原位癌早期诊断中具有实用价值。

Pathological changs and expression of placental alkaline phosphatase in testicular carcinoma-in-situ adjacent to the testicular germ cell neoplasia

Objective To observe the pathological changs and the expression of placental alkaline phosphatase (PLAP) in the testicular carcinoma_in_situ (CIS). Methods 14 cases of CIS adjacent to the testicular germ cell neoplasia were studied, and immunostaining was performed with antibody against PLAP. Results The involved seminiferous tubules showed thickned wall of the tubule, the large neoplasic cells with abundant clear cytoplasm scattered along the basement membrane, and no germ cells were seen. Immmunorstaining were found in all 14 CIS specimens, and 12 cases expressed highly PLAP. No reactivity was seen in normal germ cells and Sertoli cells. Conclusions CIS shows a characteristic histological pattern. PLAP is a sensitive, specific marker for CIS, and considerably useful in the detection of CIS.