| 靶向大鼠Caveolin-1基因的短发夹RNA表达载体的构建 |
| |
| 引用本文： | 岳扬,孙耕耘,尤青海,王楠,邵敏,张丹.靶向大鼠Caveolin-1基因的短发夹RNA表达载体的构建[J].临床肺科杂志,2012,17(5):779-780. |
| |
| 作者姓名： | 岳扬 孙耕耘 尤青海 王楠 邵敏 张丹 |
| |
| 作者单位： | 合肥,安徽医科大学第一附属医院呼吸内科,安徽,230022 |
| |
| 摘 要： | 目的构建靶向大鼠caveolin-1基因的shRNA表达载体。方法化学合成靶向caveolin-1的单核苷酸链,经退火成双链。将退火得到的双链DNA克隆至表达载体pLL3.7中得到重组质粒,经限制性内切酶酶切、DNA测序进行鉴定。结果通过限制性内切酶酶切、DNA测序证实,成功构建大鼠pLL3.7-cav-1表达载体。结论成功构建了靶向大鼠caveolin-1的shRNA表达载体,为以后进行caveolin-1与ALI/ARDS的相关研究奠定了基础。
|
| 关 键 词： | 短发夹RNA 表达载体 小窝蛋白-1 |
Construction of expression vector of short hairpin RNA targeting for caveolin-1 |
| |
| Institution: | YUE Yang,SUN Geng-Yun,YOU Qing-Hai,et al.Department of Respiratory Medicine,the First Affiliated Hospital of Anhui Medical University,Hefei,Anhui province 230022,China |
| |
| Abstract: | Objective To construct short hairpin RNA expression vector targeting for rat caveolin-1.Methods Chemosynthetic oligos were annealed and then cloned into the pLL3.7 plasmid to generate the recombinant plasmid pLL3.7-cav-1 which was confirmed by restriction endonuclease digestion and DNA sequencing.Result The vector of pLL-3.7-cav-1 was constructed successfully.Conclusion The shor hairpin RNA expression vector targeting for rat caveolin-1 has been constructed successfully,which facilitated to the study on relationship between caveolin-1 and ALI/ARDS. |
| |
| Keywords: | short hairpin RNA expression vector caveolin-1 |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
