大鼠实验性急性高眼压期间视网膜节细胞细胞色素氧化酶的组织化学研究

本文以生理盐水加压注入大鼠眼前房,二道生理记录仪监测眼内压,制成大鼠急性高眼压模型。采用改良的细胞色素氧化酶(CCO)组织化学法,研究高眼压对视网膜节细胞代谢的影响。实验动物依存活期分为0、3、7天3组,根据视网膜节细胞酶活性强度和结构变化,把CCO反应的节细胞分为3级,Ⅲ级及Ⅲ级以上的节细胞为高CCO活性细胞,作高CCO活性节细胞计数,并对各级CCO活性节细胞以显微光度计测定透光率。经自身配对t检验,表明各实验组高CCO活性节细胞数目下降;经方差分析还证明,3、7天组节细胞的酶活性有恢复趋势。对视网膜不同象限结果的比较表明,高眼压所致节细胞损害在视网膜颞侧较鼻侧更显著。

HISTOCHEMICAL INVESTIGATION OF THE CYTOCHROME OXIDASE OF RAT RETINAL GANGLION CELLS BY ACUTE EXPERIMENTAL ELEVATION OF INTRAOCULAR PRESSURE

The changes of cytochrome oxidase activity of retinal ganglion cell (RGC) caused by the artificial high intraocular pressure (HIOP) was presented in this paper. Normal saline was injected into the anterior chamber of the right eye of 36 rats. that resulted in an intraocular pressure up to 60 mmHg for 3 hours. The experimental rats were divided into 0-, 3- and 7-day groups according to their survival time. Both retinae of each rat were whole mounted on the same slide, the left one being used as control. Thirty-six pairs of retina were treated with the modified cytochrome oxidase (CCO) histochemical technique under similar conditions. The high CCO activity of RGC were counted. The extinction of the cytoplasm of RGC, which indicates the degree of CCO activity, was measured with microphotometer. As compared with the normal eyes, the high CCO activity RGC of experimental eyes were reduced significantly. It was found that the high CCO activity of RGC in the temporal side of retina has been reduced much more than that of the nasal side. However, the high CCO activity of RGC in 3- and 7-day groups were more than that of 0-day group, the extinction of CCO activity of RGC in 7-day group was lower than that of the 3-day group. These facts showed that the CCO activity might restore in various degrees followed by a longer survival time. This experiment emphasized the fact that the HIOP led to metabolic changes of RGC, which may be of value to the study of glaucoma.