| 苦参碱预处理对预防大鼠肝脏缺血再灌注损伤的作用观察 |
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| 引用本文： | 周粼,张家骅,甘平. 苦参碱预处理对预防大鼠肝脏缺血再灌注损伤的作用观察[J]. 中国现代普通外科进展, 2009, 12(7): 557-560 |
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| 作者姓名： | 周粼 张家骅 甘平 |
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| 作者单位： | 昆明医学院附属第二医院,肝胆外科,云南,昆明,650031 |
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| 摘 要： | 目的:探讨苦参碱(MT)预处理对大鼠肝脏缺血再灌注损伤的影响.方法:50只雄性SD大鼠随机分为5组:假手术组(SO组)、缺血再灌注组(NS组)和不同剂量MT(3.75、7.5、15 mg/kg)预处理组(MT1、2,3组).动物麻醉后分别经门静脉注入生理盐水或MT,然后阻断左、中叶肝蒂45 min,造成70%肝脏缺血,SO组仅行分离不阻断,再恢复供血40 min.术后采集下腔静脉血测定血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)水平,取肝组织测定超氧化物歧化酶(SOD)与丙二醛(MDA)水平,并行肝组织病理学观察.结果:MT预处理组血清ALT、AST、LDH水平及肝组织MDA含塞低于NS组,肝组织SOD含量则高于NS组;M丁预处理组肝细胞形态学异常改变较NS组明显减轻.结论:MT预处理可减轻大鼠肝脏缺血再灌注损伤,其机制可能与提高肝组织SOD含量,抑制脂质过氧化,保护线粒体有关.
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| 关 键 词： | 苫参碱 肝 缺血再灌注 大鼠 |
Effects of atrial natriuretic peptide preconditioning on hepatic ischemia-reperfusion injury in rats |
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| ZHOU Lin,ZHANG Jia-hua,GAN Ping. Effects of atrial natriuretic peptide preconditioning on hepatic ischemia-reperfusion injury in rats[J]. Chinese Journal of Current Advances in General Surgery, 2009, 12(7): 557-560 |
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| Authors: | ZHOU Lin ZHANG Jia-hua GAN Ping |
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| Affiliation: | Kunming 650031;China |
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| Abstract: | Objective:To investigate the protective effects of matrine(MT) preconditioning against hepatic ischemia-reperfusion injury in rats. Methods:Fifty male SD rats were randomly allocated into 5 groups:sham operation(SO) group,ischemia-reperfusion (NS) group and MT preconditioning groups (of 3.75,7.5,15 mg/kg). Rats of NS and MT groups were injected normal saline or MT,respectively; and 15 min later,the pedicales of the left and median liver lobes were clampped for 45 minutes,and reperfused for 40 min. The porta... |
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| Keywords: | Spragut-Dawley |
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