电针对大鼠脑出血模型海马组织氨基酸含量的影响

目的 :观察不同电针对大鼠脑出血模型海马组织氨基酸含量的影响 ,探讨电针对脑出血大鼠保护作用的可能机制。方法 :选择胶原酶加肝素联合注射法诱导脑出血大鼠模型 ,采用反相高效液相色谱荧光法观察电针不同穴位对大鼠海马氨基酸含量的影响。结果 :造模后模型组海马组织兴奋性氨基酸 (ASP、Glu)和抑制性氨基酸 (GABA)明显升高 ,存在着兴奋性氨基酸 (EAA) /抑制性氨基酸 (IAA)平衡失调。针水组能降低造模所致的Glu和GABA的升高 ,和模型组比有显著性差异 (P <0 .0 5) ,而对ASP的降低作用不明显 ,和模型组比无显著性差异 (P >0 .0 5) ;针风组能降低造模所致的ASP和Glu的升高 ,和模型组比有显著性差异 (P <0 .0 5) ,而对GABA的降低作用不明显 ,和模型组比无显著性差异 (P >0 .0 5)。结论 :针水组、针风组能抑制不同兴奋性氨基酸(Glu、ASP)的释放 ,纠正兴奋性氨基酸 (EAA) /抑制性氨基酸 (IAA)失衡 ,从而达到减轻脑出血后脑组织损害的作用。

Effect of Electroacupuncture on Changes of Amino Acid Contents in Brain Tissue of ICH Rat

Objective: To observe the effect of electroacupuncture (EA) on changes of amino acids in the ischemic cerebral hemorrhage (ICH) rat. Methods: Collagenase and heparin were injected into caudate nucleus to establish ICH rat model. The effect of EA on the content of amino acid in the brain tissue was observed. Results: The content of EAA (excitatory amino acid, ASP, Glu)and IAA(inhibitory amino acid, GABA) in the brain tissue of the model group were raised, the EAA/IAA was imbalance, the content of Glu and GABA in the brain tissue of the acupuncture "Shuigou" (DU 26) group were reduced and that was significantly compared with that of the model group(P<0.05). The contents of ASP and Glu in the brain tissue of the acupuncture "Fengfu" (DU 16) group were reduced significantly compared with that of model group (P<0.05). Conclusion:Acupuncture of"Shuigou"(DU 26) and "Fengfu"(DU 16) can reduce the release of EAA(Glu,ASP) and balance EAA/IAA, that may relieve ICH induced brain tissue injury.