亚硝胺诱发小鼠前胃鳞状细胞癌的实验研究

建立小鼠前胃鳞状细胞癌模型和观察其癌变过程。方法１００只小鼠分１０组，用肌氨酸乙酸和亚硝酸盐混和液灌胃，对照组２０只小鼠灌生理盐水，每周２次共５周。给药后每隔１０天取一实验组和２只对照组小鼠作组织学检查。结果：第２－１０组，小鼠前胃鳞状细胞 病率为１０％－４０％。

The Study on Squamous Carcinoma of Forestomach induced byNitrosamine in Mice

Objective: To establish a sqUamous carcinoma model in the forestomach of face and observe the pcness of itstnallgnant changes. Method: 100 face in 10 experimental gIDups were intubated with hared m~~ohne ethyl ester(NSEE) and odium nitrite, twice a week for 5 weeks. 20 face of the contIDI pop were intubated with NS.Histological e~nation was done in each gIDup of experimenlal animal and 2 face of contIDI gIDup et'ery 10-day afteradministration of NSEE. Result: The incidence Of sqUamous careinorna of forestomach in Zth to 10th group Ins 10% 40%. The precancerous lesions included hgnIPlasia, Papillolna, ampleal hyperplasia and careinorna in situ.COnclusion: The SqUarnous carcinoma in the forestamach Of face was induced with NSEE. The lhalignant change wasPaSSed through precancerous lesions and infilhated into submucosal layer. It is sugrsted that the nitroedne Inay be animportant factor in eitiology of the esophageal careinorna.