| 人caveolin-1基因慢病毒过表达载体构建及其对SMMC7721细胞凋亡的影响 |
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| 引用本文： | 李国平,潘润阳,张志波,何庆良,石铮.人caveolin-1基因慢病毒过表达载体构建及其对SMMC7721细胞凋亡的影响[J].福建医科大学学报,2009,43(4):297-300. |
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| 作者姓名： | 李国平 潘润阳 张志波 何庆良 石铮 |
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| 作者单位： | 1. 福建医科大学,附属第一医院病理科,福州,350005
2. 福建医科大学,附属第一医院肝胆胰外科,福州,350005 |
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| 基金项目： | 福建省自然科学基金资助项目 |
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| 摘 要： | 目的 构建人caveolin-1基因慢病毒过表达载体,并研究其对人肝细胞癌株SMMC7721细胞凋亡的影响. 方法 应用慢病毒载体系统构建人caveolin-1基因pGC-FU-caveolin-1过表达质粒,并转导SMMC7721细胞,检测其转导效率、细胞内caveolin-1 mRNA和蛋白水平的改变和对细胞凋亡的影响. 结果 pGC-FU-caveolin-1过表达质粒慢病毒转导SMMC7721细胞72 h的转导效率为(96.3±1.6)%.转导72 h和120 h后caveolin-1 mRNA水平分别是阴性慢病毒感染组的(30.15±1.22)倍和(189.16±28.13)倍,两个时相点的差别均具有统计学意义(P<0.05).同期转导后120 h的蛋白水平检测明显升高.pGC-FU-caveolin-1过表达质粒慢病毒转导SMMC7721细胞120 h后能使早期细胞凋亡数减少约70%,两者差别具有统计学意义(P<0.05). 结论 成功构建的pGC-FU-caveolin-1过表达质粒慢病毒能在SMMC7721细胞中长期稳定表达,其过表达能显著抑制SMMC7721细胞凋亡.
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| 关 键 词： | 细胞质膜微襄蛋白 癌 肝细胞 慢病毒属 遗传载体 细胞凋亡 肿瘤细胞 培养的 |
Construction of Recombinant Lentivirol Overexpression Vector Carring Human Caveolin-1 and its Correlation with Apoptosis of SMMC7721 Cell Line |
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| LI Guoping,PAN Runyang,ZHANG Zhibo,HE Qingliang,SHI Zheng.Construction of Recombinant Lentivirol Overexpression Vector Carring Human Caveolin-1 and its Correlation with Apoptosis of SMMC7721 Cell Line[J].Journal of Fujian Medical University,2009,43(4):297-300. |
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| Authors: | LI Guoping PAN Runyang ZHANG Zhibo HE Qingliang SHI Zheng |
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| Institution: | LI Guoping1,PAN Runyang2,ZHANG Zhibo2,HE Qingliang2,SHI Zheng21.Department of Pathology,The Affiliated First Hospital,Fujian Medical University,Fuzhou 3500052.Department of Hepatopancreatobiliary Surgery,Fuzhou 350005 |
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| Abstract: | Objective To construct the lentiviral overexpression vector of humon caveolin-1 and investigate its correlation with apoptosis of SMMC7721 cell line. Methods SMMC7721 cells were infected with recombinant lentivirus overexpressing human caveolin-1,and the transduction efficiency,the caveolin-1 mRNA and protein levels and the effects of caveolin-1 overexpression on the induced apoptosis of SMMC7721 cells were investigated. Results The transduction efficiency of SMMC7721 cell line with pGC-FU-caveolin-1 lentiv... |
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| Keywords: | caveolins carcinoma hepatocelluar lentivirus genetic vectors apoptois tumor cells cultured |
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