| 应用PCR技术检测恙螨体内恙虫病立克次体动态和经卵传递的研究 |
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| 引用本文： | 倪宏,陈添胜.应用PCR技术检测恙螨体内恙虫病立克次体动态和经卵传递的研究[J].寄生虫与医学昆虫学报,1994,1(2):48-53. |
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| 作者姓名： | 倪宏 陈添胜 |
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| 作者单位： | 广州中山医科大学，广州医学院附一院传染病科，广东省卫生防疫站流行病学研究所 |
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| 摘 要： | 本文应用聚合酶链反应技术检测恙虫病立克次体实验感染的恙螨体内立克次体的动态变化，通过人工腹腔接种而感染的恙螨成虫，恙虫病立克次体至少能在其体内生存３６０天和经卵传递４代；通过未食幼虫叮咬病小鼠而感染的恙螨，经饱蚴、若蛹、若虫、成蛹和成虫阶段，恙虫病立克次体检测均呈阳性，至少能在成虫期体内生存２７０天和经卵传递１代。
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| 关 键 词： | 地里纤恙螨 恙虫病立克次体 聚合酶链反应（PCR） |
STUDIES ON RICKETTSIA TSUTSUGAMUSHI PROPAGATION IN LEPTOTROMBIDIUM DELIENSE AND ITS TRANSOVARIAN TRANSMISSION BY DNA AMPLIFICATION USING POLYMERASE CHAIN REACTION TECHNOLOGY |
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| Ni Hong, Li Jiacan, Chen Tiansheng.STUDIES ON RICKETTSIA TSUTSUGAMUSHI PROPAGATION IN LEPTOTROMBIDIUM DELIENSE AND ITS TRANSOVARIAN TRANSMISSION BY DNA AMPLIFICATION USING POLYMERASE CHAIN REACTION TECHNOLOGY[J].Acta Parasitologica et Medica Entomologica Sinica,1994,1(2):48-53. |
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| Authors: | Ni Hong Li Jiacan Chen Tiansheng |
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| Abstract: | This paper reports on the detection of Rickettsia tsutsugamushi in Leptotrombidium deliense by DNA amplification using polymerase chain reaction (PCR) technology.The results indicated that R. tsutsugamushi can exist in the adult L. deliense at least 360 days by the means of inoculating R.tsutsugamushi (Karp strain) into the abdominal cavity of the mite;and it can exist at least 270 days in the body of L. deliense after bitting the infected mouse. The R. tsutsugamushi also can be transmited transovarially to the offersprings. |
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| Keywords: | Leptotrombidium deliense Rickettsia tsutsugamushi Polymerase chain reaction |
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