用中空纤维液相微萃取-HPLC法测定人血浆中酒石酸美托洛尔的浓度

目的:建立中空纤维液相微萃取-HPLC法测定人血浆中酒石酸美托洛尔的浓度.方法:优化酒石酸美托洛尔液相微萃取法供给相和接受相的浓度、萃取时间、萃取温度、萃取转速和离子强度,血浆样品经中空纤维液相微萃取法萃取后,用HPLC法测定酒石酸美托洛尔的浓度.色谱柱:Agilent Zorbax Eclipse XDB-C18柱,流动相:甲醇-0.1％磷酸(40∶60),流速:1 ml/min,激发波长:227 nm,发射波长:305 nm,柱温:30℃.结果:酒石酸美托洛尔在2～ 125 ng/ml线性关系良好,低、中、高三种浓度(5、20、100 ng/ml)的日内、日间精密度均＜10％,回收率分别为(87.1±7.3)％、(92.6±5.8)％和(89.1±2.5)％.结论:中空纤维液相微萃取-HPLC法适用于测定血浆样品中酒石酸美托洛尔的浓度.

Determination of the concentration of metoprolol tartrate in human plasma by HPLC coupled with hollow fiber liquid phase microextraction method

Objective: To establish a HPLC coupled with hollow fiber liquid phase microextraction(HF-LPME) method for determination of the concentration of metoprolol tartrate in human plasma.Methods: The concentrations of donor phase and receptive phase,time,temperature and rotary speed of extraction,and concentration of NaCl in HF-LPME were optimized.The hollow fiber was placed in plasma sample solution to perform microextraction,and then the extract was analyzed by HPLC method with fluorescence detection on Agilent Zorbax XDB-C18 column.The mobile phase consisted of methanol-0.1% phosphoric acid(40∶60) with a flow rate of 1 ml/min.The excitation wavelength was 227 nm and the emission wavelength was 305 nm.The column temperature was 30 ℃.Results: Metoprolol tartrate was in good linearity within the range of 2-125 ng/ml.The intra-day and inter-day RSD of low,middle and high concentrations(5,20,100 ng/ml) were all less than 10%,and recoveries were(87.1±7.3)%,(92.6±5.8)% and(89.1±2.5)%,respectively.Conclusion:HPLC coupled with HF-LPME method is suitable for analysis of concentration of metoprolol tartrate in human plasma.