Fractional Hepatic Localization of 14CHCl3 in Mice and Rats Treated with Chlordecone or Mirex

Fractional Hepatic Localization of ¹⁴CHCl₃ in Mice and RatsTreated with Clordecone or Mirex. Hewitt, L.A., Hewitt, W.R.,and Plaa, G.L. (1983). Fundam. Appl. Toxicol. 3:489–495.In rodents chlordecone, but not mirex, a nonketonic structuralanalog, significantly augments CHCl₃-induced liver damage, atleast in part, by increasing CHCl₃ bioactivation. To determinewhether the fractional distribution of CHCl₃ was altered inchlordecone-pretreated animals, the irreversible binding of¹⁴CHCl₃ to various liver constituents (a measure of CHCl₃ bioactivation)was examined in vivo in mice and rats. Chlordecone, but notmirex, increased both total and irreversibly bound ¹⁴CHCl₃;furthermore, changes in the ¹⁴Clocalization between lipid, proteinand acid-soluble fractions were noted. Thus, the results suggestthat differences exist between chlordecone and mirex with respectto their capacity to increase the quantity of CHCl₃-derivedreactive metabolite and the eventual distribution of reactivemetabolite.