基于抗炎和氧化应激角度研究黄连解毒汤对动脉粥样硬化大鼠作用机制

目的：研究黄连解毒汤（HuanglianJiedu Decoction，HJD）对动脉粥样硬化（atherosclerosis，AS）大鼠肝匀浆中氧化低密度脂蛋白（Ox-LDL）、单核细胞趋化蛋白（MCP-1）以及血管细胞粘附分子（VCAM-1）含量及丙二醛（MDA）、超氧化物歧化酶（SOD）活性的影响。方法：雄性SD大鼠，随机分为6组（每组10只），分别为正常对照组、模型组、阳性对照组（洛伐他汀）及低、中、高3个剂量HJD组。正常对照组喂以普通饲料，其余各组喂以高脂饲料，同时定期注射维生素D3，至第3周开始，每天灌胃给药1次，共8周，直至取材。结果：与正常对照组比较，造模后，肝匀浆MCP-1、VCAM-1、Ox-LDL含量显著升高（p<0.05），给药后，阳性对照组和HJD低、中剂量组上述指标显著下降（p<0.05），HJD高剂量组MCP-1和VCAM-1含量极显著下降（p<0.01）；肝匀浆MDA含量显著上升，给药后，阳性对照组和HJD低剂量组显著下降（p<0.05）；SOD含量显著下降，给药后，阳性组和高剂量组显著上升（p<0.05）。结论：黄连解毒汤对AS大鼠具有一定干预作用，其作用机制可能与抗炎和抗氧化反应有关。

Study on Mechanism of Huang-Lian Jie-Du Decoction on Atherosclerosis Rats Based on Anti-inflammatory and Antioxidant

This paper was aimed to study the effects of Huang-Lian Jie-Du decoction (HJD) on contents of oxidized low density lipoprotein (Ox-LDL), monocyte chemoattractant protein (MCP-1), vascular cell adhesion molecule (VCAM-1),malondialdehyde (MDA), and superoxide dismutase (SOD) of atherosclerosis (AS) rats?? liver homogenate. Male SD ratswere randomly divided into six groups, which were the normal control group, model group, positive control group(lovastatin), and the low-, middle-, high-dose HJD groups, with ten rats in each group. Except for the normal controlgroup fed with normal diet, others were fed with high-fat diet, and regularly injected the vitamin D3. All groups weregavaged once daily from the 3rd week for 8 weeks until they were sacrificed. The results showed that compared with thenormal control group, contents of MCP- 1, VCAM-1 and Ox-LDL in the liver homogenate of the model group hadsignificantly increased (p < 0.05), after drug administration, all indexes mentioned above in the positive control group, thelow- and middle-dose HJD group were decreased significantly (p < 0.05); contents of MCP-1 and VCAM-1 of the highdoseHJD group decreased obviously (p < 0.01); MDA content of liver homogenate increased significantly, after drugadministration, contents of the positive control group and the low-dose HJD group decreased significantly (p < 0.05);SOD content decreased significantly, after drug administration, contents of both the positive control group and the highdosegroup increased significantly (p < 0.05). It was concluded that HJD may play a role in AS intervention. Itsmechanism may be related to its anti-inflammatory and antioxidant reaction.