针刀干预对肌筋膜疼痛综合征模型大鼠5-HT和NE的影响

[目的] 观察针刀干预对肌筋膜疼痛综合征模型大鼠血清和触发点局部组织五羟色胺（5-HT）、去甲肾上腺素（NE）的影响，探讨针刀治疗肌筋膜疼痛综合征的机制。[方法] 将24只大鼠随机分为空白组、模型组、针刀组和注射组，每组6只。采用打击结合离心运动法建立肌筋膜疼痛综合征大鼠模型，造模8周。注射组、针刀组进行相应方法干预。用ELISA法检测各组大鼠血清及触发点局部肌肉组织5-HT、 NE的含量，采用SPSS 20.0统计学软件进行统计分析。[结果] 与空白组比较，模型组大鼠血清和局部组织5-HT、NE含量显着升高，差异有统计学意义（P<0.05），注射组大鼠血清和组织中5-HT含量显着升高（P<0.05）；与模型组比较，注射组大鼠血清5-HT、NE的含量显着下降（P<0.05），局部肌肉组织5-HT含量与之比较未见统计学差异（P>0.05），NE含量显着下降（P<0.05）；针刀组大鼠血清和局部肌肉组织5-HT、NE的含量显着下降（P<0.05）.[结论] 肌筋膜疼痛综合征模型大鼠血清和局部组织5-HT、NE含量比正常大鼠显着升高，针刀干预可以降低血清和局部组织5-HT、NE含量，注射利多卡因可以降低血清NE、5-HT及局部组织NE含量；针刀及利多卡因注射对于肌筋膜疼痛综合征的治疗作用可能与其降低血清及局部组织5-HT、NE含量有关；且针刀对于局部组织5-HT的调节作用优于药物注射。

Effect of acupotomology on 5-HT and NE in rats with myofascial pain syndrome

[Objective] To detect the concentration of 5-HT and NE in peripheral blood and the partial muscle to reveal part of the mechanism of acupuncture therapy treating myofascial pain syndrome. [Methods] The 24 rats were divided randomly and equally into control group, model group, acupunction group and injection group(lidocaine). Then we built the rat model of myofascial pain syndrome by knocking and centrifugal movement for 8 weeks.We tested the concentration of 5-HT and NE by the way of ELISA.At last,we used the program of SPSS 20.0 to do the statistical analysis. [Results] The testing result showed that the 5-HT and NE concentration of model group in the tissue and serum were significantly low than the control group(P<0.05). And the same difference with the 5-HT of injection group The 5-HT and NE concentration of acupunction group in the tissue and serum were significantly low than the model group(P<0.05). The NE concentration of injection group in the tissue and serum were significantly low than the model group(P<0.05), same as the 5-HT concentration of in the serum. But the 5-HT concentration of injection group in the tissue was not significantly different compared with the model group(P>0.05). [Conclusion] The 5-HT and NE concentration in the tissue and serum of the myofascial pain syndrome model rat are significantly low than the normal rat.The acupunction therapy can reduce the 5-HT and NE concentration in the tissue and serum. Injection therapy can reduce the 5-HT concentration in serum and the NE concentration in the tissue and serum. But can not reduce the 5-HT concentration in tissue. So reducing the concentration of 5-HT and NE may be one of the treatment mechanisms of acupunction therapy. And the effect of reducing 5-HT concentration in the tissue of acupunction therapy than that of drug therapy.