电针对吗啡成瘾大鼠中枢多巴胺能神经元的影响

目的:探讨吗啡对大鼠中脑腹侧被盖区(VTA)、伏隔核(NAc)、前额皮层(PFC)内酪氨酸羟化酶(TH)及胶质纤维酸性蛋白(GFAP)的影响及电针的调节作用。方法:大鼠随机分为正常对照组、模型组、电针治疗组,每组5只,模型组、电针治疗组采用吗啡自身给药成瘾大鼠模型。电针治疗组选取双侧L5、L2夹脊穴,20 min/d,电针连续治疗10 d;正常对照组、模型组不予电针干预。采用免疫组化法观察各组大鼠VTA、NAc、PFC内TH、GFAP含量的变化。结果:与正常对照组比较,模型组大鼠脑区VTA、NAc、PFC内TH及VTA内GFAP含量明显增加,差异均有统计学意义(P0.05);与模型组比较,电针治疗组大鼠脑区VTA、NAc、PFC内TH及VTA内GFAP含量明显降低,差异均有统计学意义(P0.05)。结论:吗啡成瘾后大鼠相关脑区的多巴胺能神经元发生了相应改变,电针治疗可调节多巴胺能神经元的异常表达,对吗啡成瘾大鼠的多巴胺能神经元起到保护作用。

Effects of Electroacupuncture on Central Dopaminergic Neuron in Morphine Addicted Rats

  Objective:To investigate the effects of morphine on tyrosine hydroxylase(TH)and glial fibrillary acidic protein(GFAP)in the ventral tegmental area(VTA),nucleus accumbens(NAc)and prefrontal cortex(PFC)of rats,and discuss the regulation effects of electroacupuncture.   Methods: The rats were randomly divided into the normal control group,model group and electroacupuncture treatment group,5 rats in each group. The addicted rat model was established by self-medication of morphine in the model group and electroacupuncture treatment group. The electroacupuncture treatment group was treated with electroacupuncture on Jiaji(EX-B2) acupoint of bilateral L5 and L2 segments,with a course of 20 minutes every day for continuous 10 days. The normal control group and model group were given no electroacupuncture intervention. The contents of TH and GFAP in VTA,NAc and PFC of rats in each group were detected by immunohistochemistry.   Results: Compared with the normal control group,the content of TH in VTA,NAc and PFC of brain and the content of GFAP in VTA were obviously increased in the model group,with statistically significant differences(P<0.05). Compared with the model group,the content of TH in VTA,NAc and PFC of brain and the content of GFAP in VTA were obviously decreased in the electroacupuncture treatment group,with statistically significant differences(P<0.05).   Conclusion: After morphine addiction,the dopaminergic neurons in the brain related regions of rats changed accordingly. The therapy of electroacupuncture can regulate the abnormal expression of dopaminergic neurons,which shows the protective effects on dopaminergic neurons in morphine addicted rats.