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| 白藜芦醇对UVB损伤HaCaT细胞保护作用的双向电泳图谱差异分析 | |
| 引用本文： | 王毅铮,张勇,王瑜,高宏生,赵化冰,崔自由,陈虹.白藜芦醇对UVB损伤HaCaT细胞保护作用的双向电泳图谱差异分析[J].卫生研究,2011,40(5). |
| 作者姓名： | 王毅铮 张勇 王瑜 高宏生 赵化冰 崔自由 陈虹 |
| 作者单位： | 1. 武警医学院天津市职业与环境危害生物标志物重点实验室,天津,300162 2. 武警8620部队医院药械科 3. 武警总医院医务科 |
| 摘 要： | 目的利用双向电泳技术(2-DE)从蛋白质组学角度分析白藜芦醇对中波紫外线(UVB)照射的人角质形成细胞(HaCaT细胞)的保护作用,以探讨白藜芦醇防御UVB损伤的作用靶点及有关机制。方法 MTT法检测不同浓度的白藜芦醇对经或不经UVB照射的HaCaT细胞增殖的影响。2-DE实验分对照组、UVB组、白藜芦醇组、UVB+白藜芦醇干预组(共同干预组),提取各组蛋白进行2-DE实验,结合质谱(MALDI-TOF-MS)分析与数据库检索对差异蛋白质点进行鉴定。结果筛选10个存在明显差异的蛋白点,质谱鉴定出8种蛋白质,除4种功能未知蛋白外,其余4种蛋白分别为转录起始因子IIE-β(TFIIE-β)、组蛋白H1.2、锌指蛋白、MAGOH蛋白。UVB组细胞内组蛋白H1.2水平较其它各组明显增高(P<0.05);UVB组、UVB+白藜芦醇干预组的TFIIE-β及锌指蛋白表达水平均降低,UVB组下降最为明显(P<0.05);UVB组的MAGOH蛋白较各组明显减少(P<0.05)。结论应用2-DE联合质谱技术发现了白藜芦醇对UVB诱导的HaCaT细胞损伤防护作用相关的差异蛋白点。 |
| 关 键 词： | 双向电泳 白藜芦醇 紫外线 人角质形成细胞 |
Differential analysis of two-dimensional gel electrophoresis profiles in the protective efffects of resveratrol on HaCaT cells induced by UVB irradiation |
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| WANG Yizheng,ZHANG Yong,WANG Yu,GAO Hongsheng,ZHAO Huabing,CUI Ziyou,CHEN Hong.Differential analysis of two-dimensional gel electrophoresis profiles in the protective efffects of resveratrol on HaCaT cells induced by UVB irradiation[J].Journal of Hygiene Research,2011,40(5). | |
| Authors: | WANG Yizheng ZHANG Yong WANG Yu GAO Hongsheng ZHAO Huabing CUI Ziyou CHEN Hong |
| Institution: | WANG Yizheng,ZHANG Yong,WANG Yu,GAO Hongsheng,ZHAO Huabing,CUI Ziyou,CHEN Hong Tianjin Key Laboratory of Biomarkers for Occupational and Environmental Hazard,Medical College of Chinese People's Armed Police Forces,Tianjin 300162,China |
| Abstract: | Objective To analysis the differences of protein expression and possible protective mechanisms of resveratrol(Res) on human keratinocytes(HaCaT cells) irradiated by ultraviolet B(UVB).Methods MTT test was used to assay the effects of Res on the proliferation of HaCaT cells.The tested objects were divided into four groups,the control,UVB irradiation,Res intervention and UVB + Res intervention(r-u) groups.Then the protein of cells in the four groups was separated by a two-dimensional gel electrophoresis(2-DE)... |
| Keywords: | two-dimensional gel electrophoresis resveratrol ultraviolet b HaCaT cells |
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