甲基丙烯酸环氧丙酯致人支气管上皮细胞染色体损伤的研究

目的研究甲基丙烯酸环氧丙酯(GMA)对人支气管上皮(16HBE)细胞染色体损伤的情况,探讨其潜在致癌性。方法 GMA染毒16HBE细胞后,收获不同染毒剂量(4、8、12、16、20μg/ml)、染毒次数(1、2、3次)、转化代数(10、30代)的细胞进行染色体畸变分析。结果在4～20μg/ml剂量范围内,随染毒剂量的增加,染色体畸变率(3%、6%、7%、11%、14%)升高,并呈剂量-反应关系;随着染毒次数增加,3次染毒的细胞染色体畸变率(6%、7%、10%)显著增高;转化第10代细胞表现为染色体结构畸变,而转化第30代细胞主要表现为染色体数目畸变。结论 GMA能够诱导16HBE细胞染色体畸变,且畸变类型由早期的结构畸变转变为数目畸变。

Chromosome damage of human bronchial epithelial cells induced by glycidyl methacrylate

Objective To observed the chromosome damage of human bronchial epithelial cells induced by glycidyl methacrylate(GMA).Methods Chromosome aberration analysis of human bronchial epithelial cells treated with GMA at different dosages(4,8,12,16 and 20μg/ml),times(1,2 and 3 times),and phases(10th,30th genetation) was detected.Results In the dosages range from 4 to 20μg/ml,the aberration rates(3%,6%,7%,11% and 14%) were demonstrated increasingly with the increase of exposure doses,and dose-effect relationship was found.Significant differences were observed when treated with GMA three times(6%,7% and 10%).Structure aberrations were found in the transformed 10th-generation cells,while number aberrations were mainly manifested in the transformed 30th-generation cells.Conclusion The chromosome aberration can be induced by GMA in the human bronchial epithelial cells,from the structure aberration at the beginning to the lack of normal nuclear style.