六种哺乳类动物肝细胞内溶酶体形态的比较研究

本文用电镜、酸性磷酸酶ACPase组化和立体计量分析,对成年猴、狗、猫、兔、大鼠和小鼠肝细胞内溶酶体作了超微结构定性和定量的比较观测。 几种动物肝细胞的致密体超微结构相似,但数量和大小各有差别。消化泡以大鼠、小鼠、兔和猴的数量最多,狗和猫的较少。与此相反,大鼠、小鼠和兔的殁体较少见,猫和猴的稍多,狗的残体为数最多,且见有在其旁的未消化物质正进入残体内进行消化。这几种哺乳类动物肝细胞内消化泡与残体所出现的差别,据推测可能与所食饲料的性质和动物种属不同有关。 鬃毛有衣小泡多位于细胞膜的深处。在狗和猴见到侧邻的两个肝细胞的一侧胞膜出芽,陷入胞质内,其末端膨大,似脱离而形成鬃毛有衣小泡。 6种哺乳类动物肝细胞内溶酶体的形态计量分析所得结果;体积密度中,以狗为最高,小鼠最低;数目密度中,猴最高,兔最低。

A Comparative Study of Hepatocyte Lysosomes in Six Kinds of Mammals

A qualitative and quantitative comparison of the ultrastructure of the hepato-cyte lysosomes of adult monkeys, dogs, cats, rabbits, rats, and mice were carried out.The dense bodies in the hepatocytes in the animals studied were similar in their ultrastructure but different in their number and size. The digestive vacuole was more numerous in rats, mice, rabbits and monkeys than in dogs and cats. But the residue body was scarcely found in rats, mice and rabbits, a little more in cats and monkeys, and quite a lot in dogs. Besides, some undigested globular substances were found just entering into the residue bodies to be digested. The difference of the digestive vacuoles and residue bodies in the hepatocytes of these mammals are probably due to the species difference and the different kinds of food eaten.In dogs and monkeys, bristle coated vesicles were formed by the budding out of the adjacent cell membrane of two hepatocytes with one end of the budding in-vaginating into the cytoplasm and the other end enlarged and pinching out.The results of morphometric analysis of lysosomes of the hepatocytes were different in the 6 kinds of mammals studied.The volume density of lysosomes of the hepatocytes was the highest in dogs and the lowest in mice, and the numerical density was the highest in monkeys and the lowest in rabbits.