一种腹腔镜人工气腹体外模型的建立及评价

目的：建立腹腔镜人工气腹体外模型并评价其效果。方法：采用不锈钢密封罐和自动气腹机建立腹腔镜人工气腹体外模型，用M1Tr法观察CO2气腹（7mmHg，4h）对人宫颈癌细胞CaSki体外生长的影响，实验重复3次。设未处理组为对照。结果：CO2气腹组和对照组同次处理的组间比较结果显示，处理后第24h、48h两组吸光度值无明显差异（P〉0．05），处理后第72h、96h、120hCO2组各时间点吸光度值明显高于对照组（P〈0．05）。两组组内3次重复实验同一时间点所测吸光度值（A）无显著差异（P〉0．05）。结论：采用密封罐和自动气腹机建立的腹腔镜人工气腹体外模型是一种进行腹腔镜体外研究的理想模型；采用该模型发现CO2气腹促进人宫颈癌细胞CaSki的体外生长。

Establishment and assessment of a laparoscopic pneumoperitoneum model in vitro

Objective:To establish and assess a laparoscopic pneumoperitoneum model in vitro.Methods:The model was established by a special airtight container made of stainless steel and an automatic pneumoperitoneum meter.After the cervical carcinoma cell line(CaSki) was exposed to 100% carbon dioxide in this model,the effect of carbon dioxide pneumoperitoneum on the cell growth was determined with MTT assay.And the experiment was repeated for three times.Results:There was no significant difference of the absorbance values at 24h?48h after exposure between the two groups(P>0.05),but at 72h,96h,120h after exposure,the absorbance values in carbon dioxide pneumoperitoneum group were always significantly higher than those in control group at the same time(P<0.05).There was no significant difference of the change of the absorbance values either in the carbon dioxide pneumoperitoneum group or in the control group among three times(P>0.05).Conclusions:The model established with a special airtight container and an automatic pneumoperitoneum meter is an ideal one for research about laparoscopy in vitro,and the carbon dioxide pneumoperitoneum in this model increases the in vitro growth of human cervical carcinoma cells.