灰色体系中H-point法的应用研究

目的：研究广义H-point法在灰色体系中的应用，考察其分析结果的可靠性。方法：扫描槐米和芦丁的紫外吸收光谱，优化纯组分区间，利用标准加入系列光谱数据，测定主要成分芦丁的含量，并与药典方法比较。结果：该法回收率为100.6%，RSD=3.11%（n=7），芦丁的含量为16.17%，RSD=3.37%(n=5)；药典方法测得量为17.24%，RSD=4.95%(n=5），两无显性差异。结论：此法适合于灰色体系的分析，为快速分析中药提供了一条重要思路，具有一定的实际应用价值。

Study of H-point Methods Applied to Gray Systems Analysis

AIM: The purpose is to study the precision and accuracy of H- point methods applied to gray systems analysis. METHODS: Study the UV spectra of Flos Sophorae to select the wavelength ranges without disturbing of interference and to determin the content of rvt.in to compared with pharmacopeia methods. RESULTS: The recovery by means of this method is 100.6%. RSD=3.11% (n=7) ; The content of rutin of the sample is 16.17%, RSD=3.37% (n= 5); The content of sample obtained by pharmacopeia method is 17.24%, RSD=4.95% (n= 5),There is no significant difference between the two methods. CONCLUSION:This method can be used to deal with gray systems quickly like traditional and herbal drugs.