牛蛙初级感觉神经P物质对GABA激活电流的抑制作用(英文)

目的　在牛蛙背根神经节神经元研究P物质对γ -氨基丁酸激活电流的调制作用。方法　实验采用的方法是全细胞膜片钳技术 ,实验材料为急性分离的牛蛙背根神经节神经元。结果　在受检的神经元中大多数神经元 ( 95% )对γ -氨基丁酸 ( 1～ 1 0 0 0um)敏感 ,并且能被荷包牡丹碱 (GABA受体的阻断剂 )所阻断。另一方面 ,P物质能抑制γ -氨基丁酸激活电流的 3 0 .85%。结论　由于P物质受体和γ -氨基丁酸受体共存于牛蛙DGR神经元膜 ,并且P物质受体的激活可以调制γ -氨基丁酸受体激活的反应 ,从背角神经元释放的P物质可以逆转由γ -氨基丁酸介导的突触前抑制反应

Inhibition of substance P of primary sensory neurons on

Objective To research the inhibiting effect of substance P of dossal root ganglion(DRG) in bullfrog neurons on GABA-activated currents.Method The experiment was carried out in freshly isolated bullfrog using a whole-cell patch-clamp technique.Results The majority of the neurons examined(95/100)were sensitive to GABA(1～1000μM)and can be blocked by bic ( bicuculline, GABA A receptor antagonist).Substance P inhibited the GABA-activated currents to 30.85%.Conclusion Since the SP receptor and GABA receptor can coexist in bullfrog DRG neurons and activation of SP receptor can modulate the response mediated by GABA receptor, it suggests that SP released from dorsal horn neurons can reverse presynaptic inhibition mediated by GABA.