系统性红斑狼疮患者血清IL-10的水平及临床意义

目的 观察系统性红斑狼疮(systemic lupus erythematosus,SLE)患者血清IL-10的表达与疾病活动的关系.方法 选取22例SLE患者及24名健康人作为对照,根据狼疮疾病活动指数(SLE disease activity index,SLEDAI)将SLE患者分为活动期组和非活动期组,检测血清抗dsDNA抗体,血清总补体溶血活性(CH50)及C反应蛋白(C reactive protein,CRP),酶联免疫吸附法(ELISA)检测血清IL-10表达.结果 与对照组[(18.11±6.97)ng/L]相比,IL-10在SLE活动期组[(78.54±5.62)ng/L,P＜0.01]及非活动期组[(30.36±10.98)ng/L,P＜0.05]均有所增高,活动期组增高更为明显(与非活动期组相比,P＜0.05).IL-10水平与SLEDAI呈正相关(SLE活动期,r=0.77,P＜0.01;SLE非活动期,r=0.84,P＜0.01),IL-10的水平与抗dsDNA抗体(r=0.71,P＜0.01)、CRP(r=0.63,P＜0.01)和CH50(r=-0.56,P＜0.05)均相关.结论 IL-10在SLE患者血清中表达升高,在疾病活动时更为明显,IL-10能反应疾病活动的程度,可以做为临床观察SLE疾病活动的指标之一.

Correlation between serum IL-10 and clinical value in patients with systemic lupus erythematosus

Objective To investigate the correlation between IL-10 and disease activity in patients with systemic lupus erythematosus(SLE).Methods According to the SLE disease activity index(SLEDAI),22 SLE patients divided into two groups:activity phase group(n=12)and inactivity phase group(n=10),24 healthy subjects as control.Serum IL-10 in patients and control group was measured by enzyme linked immunosorbent assay.The correlation of serum IL-10 with SLEDAI,anti-double strain DNA(dsDNA)antibody,C reactive protein(CRP),50%complement hemolytic activity(CH50)was analyzed.Results Compared with the control group[(18.11±6.97)ng/L],serum IL-10 level was significantly higher in SLE patients,no matter in activity phase[(78.54±5.62)ng/L,P＜0.01]or inactivity phase[(30.36±10.98)ng/L,P＜0.05],and significant difference was also observed between activity and inactivity phase(P＜0.05).Significant correlations between serum IL-10 concentration and SLEDAI(activity phase group,r=0.77,P＜0.01;inactivrty phase grap,r=0.84,P＜0.01),serum anti-dsDNA antibody(r=0.71,P＜0.01),CRP(r=0.63,P＜0.01),CH50(r=-0.56,P＜0.05)were found.Conclusion Serum IL-10concentration is increased in SLE patients and correlates with SLE disease activity.Detection of serum IL-10 levels seemed to be a useful index for monitoring disease activity in SLE patients.