替加色罗对肝硬化大鼠肠传输功能的影响

目的研究替加色罗对肝硬化大鼠肠道传输功能的影响,探讨其可能的作用机制。方法24只Wister大鼠随机分为肝硬化模型组、肝硬化替加色罗组和正常对照组,采用葡聚糖蓝-2000为胃肠内标记物,观察大鼠肠道传输的变化,同时放射免疫测定大鼠血浆：肠道组织中胃动素（MTL）、血管活性肠肽（VIP）及P物质（SP）含量的变化,乙酰胆碱酯酶（Ache）组织化学染色,观察肝硬化大鼠小肠肌间神经丛胆碱能神经的变化,并对其分布进行定量分析。结果与对照组比较,肝硬化模型组大鼠小肠动力显著减弱（P〈0.01）,血浆及空肠组织中MTL、VIP的含量明显增加,SP的含量则显著减少（P〈0.01）,小肠肌间神经丛胆碱能神经的分布明显减少（P〈0.01）;肝硬化替加色罗组大鼠小肠动力与对照组比较无明显差异,血浆及空肠组织中MTL、VIP的含量与模型组比较无明显差异,SP的含量及肠肌间神经丛胆碱能神经的分布则与肝硬化模型组差异显著（P〈0.01）。结论替加色罗对肝硬化大鼠小肠运动功能减退有明显改善作用,其机制可能与SP及肌间神经丛胆碱能神经的分布变化有关,而MTL、VIP可能未参与其作用。

The effects of tegaserod on interstinal motility in rats with cirrhosis

Objective To observe the effects of tegaserod on intestinal motility in rats with cirrhosis and to discover its mechanism.Methods Twenty four Wister rats were randomly divided into cirrhosis model group, cirrhosis treated with tegaserod group and control group, and the intestinal motility changes of rats were assayed by Dextran blue-2000 as a indicator. The contents of motilin(MTL), substance P (SP)and vasoactive intestinal peptide (VIP) in plasma and jejunum tissue were determined with radioimmunonassay. The cholinergic nerves in jejunal myoenteric plexus were observed with acetylcholinesterase histochemical staining and analysed by computer. Resultes Compared with control group, intestinal motility of rats in cirrhosis model group was markedly delayed(P<0.01), the contents of MTL and VIP were markedly increased (P<0.01,P<0.05) in plasma and jejunum, SP was markedly decreased (P<0.001), and the distributions of myenteric cholinergic nerves were significantly decreased. In tegaserod group, the intestinal motility of rats was significantly recovered, the contents of MTL and VIP had no markedly changes compared with model group, and the SP content and the distributions of myenteric cholinergic nerves had markedly changes. Conclusion Tegaserod may improves intestinal motility of rats with cirrhosis, which may be associated with SP and myoenteric cholinergic nerves.