不同程度听力损失动物模型的快速制备

通过一定剂量的卡那霉素与不同剂量的利尿酸钠联合应用，使猫在短时间内出现不同程度的听力损失。将19只听力正常猫随机分成三组。第一组：肌注卡那霉素（300mg/kg)半小时后，以1mg/min的速度静脉推注利尿酸钠，同时记录ABR以连续观察听力情况，当阈值开始提高时，停止用药。第二、三组：减少利尿酸钠用量，分别为第一组的1／3、2／3，其余步骤同第一组。结果显示：第一组在短时间内出现严重的不可逆的听力损失；第二、第三组出现轻、中度听力损失。这为听力学基础和临床研究快速制备了不同程度耳聋的动物模型。

The Rapid Development of Animal Models of Various Deaf Degrees

Co-administration of kanamycin(KA) with the loop diuretic ethacryni cacid(EA) has previously been shown to produce a rapid and profound hearing loss.In the present study we use a modified technique for developing hearing losses to various degrees in cats.19 cats were randomly divided into three groups freely.The first group received an intramuscular injection of KA(300 mg/kg) followed by intravenous injection of EA(1 mg/min).Click-evoked auditory brainstem responses(ABRs) were recorded to monitor the animal's hearing during the injection. when the ABR thresholds began to rise the infusion of EA was stopped.The second and third group,after the injection of KA,received intravenous injection of EA(300mg/min),the doses of which were 1/3 and2/3 times those of the first group respectively.The results are as follws the first group were inflicted with a profound hearing loss in a shorit time;the second and third group were inflicted with profound hearing losses of low and middle degrees.This technique provides an appropriate way to rapidly create animal models of various deaf degrees.