| 用ELISA检测流行性出血热特异性免疫复合物的初步研究 |
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| 引用本文： | 喻植群,杨东亮.用ELISA检测流行性出血热特异性免疫复合物的初步研究[J].现代免疫学,1993,13(6):359-361. |
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| 作者姓名： | 喻植群 杨东亮 |
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| 作者单位： | 同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院临床免疫研究室，同济医科大学附属同济医院临床免疫研究室 武汉 430030，武汉 430030，武汉 430030，武汉 430030，武汉 430030 |
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| 摘 要： | 为检测流行性出血热特异性循环免疫复合物,建立了抗原特异性双抗体夹心ELISA技术。以兔抗EHF IgG包被反应板,使待检标本内循环免疫复合物通过暴露的EHF病毒抗原位点而被结合到固相上,再以辣根过氧化物酶标记羊抗人IgG进行示踪,从而检出EHF’特异性循环免疫复合物。在228份EHF患者血清中,用胶因素ELISA检出非特异性循环免疫复合物183份,抗原特异性ELISA检出特异性循环免疫复合物188份。本方法特异性强,重复性及稳定性较好,可应用于临床检测。
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| 关 键 词： | 流行性出血热 病毒抗原 循环免疫复合物 |
DETECTION OF EPIDEMIC HEMMORRHAGIC FEVER SPECIFIC CIRCULATING IMMUNE COMPLEXES BY ELISA |
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| Yu Zhiqun Yang Dongliang Li Fanghe Chen Yue Hao LianjieClinical Immunology Research Unit,Tongji Hospital,Tongji Medical University.DETECTION OF EPIDEMIC HEMMORRHAGIC FEVER SPECIFIC CIRCULATING IMMUNE COMPLEXES BY ELISA[J].Current Immunology,1993,13(6):359-361. |
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| Authors: | Yu Zhiqun Yang Dongliang Li Fanghe Chen Yue Hao LianjieClinical Immunology Research Unit Tongji Hospital Tongji Medical University |
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| Institution: | Wuhan 430030 |
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| Abstract: | An antigen-specific method has been developed for the direct detection of EHF circulating immune complexes. Rabbit anti-EHF coated microtitre plates were incubated with the CIC containing samples. The EHF antigen containing CIC-were bound to the solid phase by their free antigenic determinants and could be detected with a peroxidase-labelled anti-human IgG antibody. In 228 sera samples of EHF patients, 183 samples were positive for CIC by conventional conglutinin-ELISA. EHF-CIC was detected in 188 samples by the present antigen-specific method. The two correlated well, however,the conventional method was also positive in serum from chronic hepatitis and other auto-immune diseases due to its non-specificity. |
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| Keywords: | epidemic hemmorrhagic fever virus antigen circulating im-mune complexes |
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