| 姜黄素对结肠癌SW-480细胞抗失巢凋亡的影响 |
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| 引用本文： | 许则丰,范钰,丁佳逸. 姜黄素对结肠癌SW-480细胞抗失巢凋亡的影响[J]. 中国中医基础医学杂志, 2006, 12(3): 187-189 |
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| 作者姓名： | 许则丰 范钰 丁佳逸 |
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| 作者单位： | 1. 浙江大学医学院附属第二医院浙江大学,肿瘤研究所,浙江,杭州,310009
2. 浙江省新和成股份有限公司,浙江,新昌,312500 |
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| 摘 要： | 目的:探讨姜黄素对结肠癌细胞抗失巢凋亡的影响。方法:采用不同浓度的姜黄素处理结肠癌SW-480细胞,采用末端脱氧核苷酸转移酶介导的dUTP切口末端标记法(TUNEL)法检测结肠癌细胞抗失巢凋亡,采用软琼脂集落形成试验检测细胞的生长增殖。结果:姜黄素能有效地抑制结肠癌SW-480细胞锚着不依赖性增殖,且呈浓度依赖性。结肠癌细胞经姜黄素处理后,可促进失巢凋亡且与时间和浓度相关。结论:姜黄素可有效地抑制结肠癌细胞锚着不依赖性增殖,其机制可能与促进失巢凋亡有关。
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| 关 键 词： | 结肠癌 姜黄素 失巢凋亡 |
| 文章编号： | 1006-3250（2006）03-0187-03 |
| 收稿时间： | 2005-01-22 |
| 修稿时间： | 2005-01-22 |
Effects of Curcumin on Anoikis Resistance of Human Colon Cancer Cell SW-480 |
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| XU Ze-feng,FAN Yu,DING Jia-yi. Effects of Curcumin on Anoikis Resistance of Human Colon Cancer Cell SW-480[J]. Chinese Journal of Basic Medicine In Traditional Chinese Medicine, 2006, 12(3): 187-189 |
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| Authors: | XU Ze-feng FAN Yu DING Jia-yi |
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| Affiliation: | 1. Cancer institute, Second hospital of Zhejiang University College of Medcine, Hangzhou 310009, China; 2. Zhejiang NHU Company Ltd., Xinchang 312500, China |
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| Abstract: | Objective: To study the effects of curcumin on anoikis resistance in human colon cancer cells.Methods: After colon cancer SW-480 cells were treated with curcumin,the growth of anchorage independence of colon cancer SW-480 cells was examined in soft agar colony formation,and apoptosis of colon cancer were evaluated by terminal deoxynucleatidyl transferase-mediated deoxyuridine triphosphate nick end labeling(TUNEL) and DNA ladder.Results: Curcumin could significantly inhibit the growth of anchorage independence of colon cancer SW-480 cells in soft agar colon formation in a dose-dependent. Curcumin could induce apoptosis of colon cancer cells from DNA ladder and TUNEL assay,which were in a timeand dose-dependent manner.Conclusions: Curcumin could inhibit the growth of anchorage independence of colon cancer SW-480 cells,which was related to anoikis. |
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| Keywords: | colon cancer curcumin anoikis |
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