| HPLC测定5-FU方法中内标物对氨基苯甲酸的研究 |
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| 引用本文： | 王桥,宋学英,张枫,朱莹. HPLC测定5-FU方法中内标物对氨基苯甲酸的研究[J]. 首都医科大学学报, 2003, 24(3): 237-239 |
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| 作者姓名： | 王桥 宋学英 张枫 朱莹 |
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| 作者单位： | 首都医科大学化学教研室(王桥，宋学英，张枫)，首都医科大学化学教研室(朱莹) |
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| 基金项目： | 北京市留学人员科技活动择优资助项目 |
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| 摘 要： | 为改进HPLC测定氟尿嘧啶 (5 fluorouracil,5 FU)的方法 ,选择对氨基苯甲酸 (PABA)为内标物 ,用C18色谱柱 ,流动相甲醇 磷酸缓冲液 (pH 6.8) ,检测波长 2 70nm ,测定血清中 5 FU含量。结果 5 FU的最小检出限量为 1 .2ng,5 FU及PABA的萃取回收率分别大于 88.0 1 %和 86.5 5 %。
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| 关 键 词： | 高效液相色谱法 氟尿嘧啶 对氨基苯甲酸 |
| 收稿时间： | 2003-01-03 |
| 修稿时间： | 2003-01-03 |
PABA as Internal Standard in Method of HPLC for Determining 5-FU Concentration in Serum |
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| Wang Qiao,Song Xunying,Zhang Feng,Zhu Ying. PABA as Internal Standard in Method of HPLC for Determining 5-FU Concentration in Serum[J]. Journal of Capital Medical University, 2003, 24(3): 237-239 |
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| Authors: | Wang Qiao Song Xunying Zhang Feng Zhu Ying |
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| Affiliation: | Wang Qiao,Song Xunying,Zhang Feng,Zhu Ying Department of Chemistry,Capital University of Medical Sciences |
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| Abstract: | To improve the determination of 5-FU by high-performance liquid chromatography, PABA was selected as an internal standard in the analytical method. For determination of 5-FU concentration in serum, a reversed-phase C 18 column with a mobile phase consisting methanol-phosphate buffer (pH 6.8) was used. UV detector was set at 270 nm. The limit of determination for 5-FU was 1.2 ng. The extraction recovery of 5-FU and PABA were more than 88.01% and 86.55% respectively. |
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| Keywords: | HPLC 5-fluorouracil p-aminobenzonic acid(PABA) |
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