| 乙型肝炎病毒蛋白对核酶剪切作用的影响 |
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| 引用本文： | 连建奇,周永兴,王九平,金由辛.乙型肝炎病毒蛋白对核酶剪切作用的影响[J].第四军医大学学报,2000,21(7):784-786. |
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| 作者姓名： | 连建奇 周永兴 王九平 金由辛 |
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| 作者单位： | 1. 第四军医大学唐都医院,感染科,陕西,西安,710038
2. 中国科学院上海生化所,上海,200031 |
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| 基金项目： | 国家自然科学基金!资助项目 ( 3 95 70 65 2 ) |
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| 摘 要： | 惭型肝炎病毒蛋白对核酶剪切作用的影响。方法 利用计算机辅助设计针对乙型肝炎病毒Ｃ基因的三个单一核酶构建Ｒｚ１核酶自剪切转录载体（ｐＧＥＭＲｚ１），观察单一核酶（Ｒｚ１）对靶ＲＮＡ的切割作用及乙型肝炎病毒观察单一核酶（Ｒｚ１）对靶ＲＮＡ的切割作用及乙型肝炎病毒蛋白对核酶剪切作用的影响。结果 核酶自剪切转录载体体２上转录后，在顺式核酶发生自剪切后可将目的核酶正确地释放出来，计算机设计的单一核酶体外可剪
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| 关 键 词： | 乙型肝炎病毒 核酶 病毒蛋白 基因表达 |
Protein action of ribozyme-mediated cleavage on hepatitis B virus in vitro |
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| LIAN Jian-Qi,ZHOU Yong-Xing,WANG Jiu-Ping,JIN You-Xin.Protein action of ribozyme-mediated cleavage on hepatitis B virus in vitro[J].Journal of the Fourth Military Medical University,2000,21(7):784-786. |
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| Authors: | LIAN Jian-Qi ZHOU Yong-Xing WANG Jiu-Ping JIN You-Xin |
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| Abstract: | AIM To detect HBV protein influence on the cleavage activity of ribozyme. METHODS Three single ribozymes against hepatitis B virus core gene were designed with computer, then transctiptional vector of three singleribozymes were constructed, HBV protein influence on the cleavage activity of single ribozyme on target RNA observed, and HBV protein interaction with target RNA further analysed. RESULTS Single antiviral ribozyme(Rz1) could cleave its target RNA efficiently, HBV proteins had no effects on ribozyme mediated cleavage on HBV. Further results indicated that HBV proteins did not interact with target RNA in our experiment. CONCLUSION HBV proteins has no effects on ribozyme mediated cleavage on HBV. |
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| Keywords: | hepatitis B virus ribozyme viral protein gene expression |
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