大鼠嗅球中凋亡相关基因蛋白和成纤维细胞因子表达的相关性及其意义

目的　明确成纤维细胞因子 (fibroblastgrowthfactor,FGF)和凋亡相关基因Bcl 2及Bax在大鼠嗅球中的表达及相关性 ,并探讨老化性嗅觉障碍的机制。方法　取健康大鼠青龄组 ( 3个月 ) ,老龄组 ( 2 4个月 )各 10只 ,断头处死 ,取嗅球 ,经石蜡包埋 ,连续切片 ,免疫组化染色 ,光镜观察。结果青龄组大鼠嗅球中Bcl 2和FGF阳性细胞表达率分别为 ( 86 40± 5 2 2 ) %和 ( 70 2 5± 6 0 1) % ,显著高于老龄组的 ( 30 0 0± 4 12 ) %和 ( 2 4 10± 4 0 0 ) % ,二者呈明显正相关 ( x±s,P <0 .0 1,r =0 .8971) ;Bax阳性细胞表达率老龄组为 ( 98.2 4± 6 .5 0 ) % ,虽高于青龄组的 ( 82 .2 5± 6 .37) % ,但差异不显著。结论　随着年龄的增长嗅球中FGF表达减少 ,抗凋亡基因Bcl 2明显减少 ,提示嗅球中抗凋亡基因Bcl 2表达受成纤维细胞生长因子的调控 ,且与老化密切相关。

Expression of apoptotic gene and FGF on olfactory bulb of rat and its significance

Objective To study the expression and distribution of apoptotic gene Bcl 2 and Bax on olfactory of rat , and its relation with the FGF and to investigate the mechanism of senile dysosmia.Methods Ten young (3 months) and ten senile rats (24 months) were used in this study. After the removal of the heads of these rats, olfactory bulb were immediately fixed with neutralized formalin, followed with paraffin embedding, serial sectioning, immunohistochemiscal staining and microscopic observing. Results The positive expression rate of Bcl 2 and FGF on olfactory bulb in young rats was significantly stronger than that in senile rats ( P <0.01). The positive expression rate of Bax in senile rats was a little stronger than that in young rats, but the differences were not statistically significant. ( P >0.05) Conclusion The expression of FGF and anti apoptotic gene Bcl 2 on olfactory bulb decrease with the increase of age. This suggests neuron apoptosis on olfactoy bulb are regulated by neurotrophic factor, and directly related with senile dysosmia. The mitral cell apoptosis as a result of FGF decreased on olfactory bulb may play a key role in the senile dysosmia .