The Expression of MMP—3 and TIMP—1 During Orthodontic Periodontium Remodeling in Rats

目的：观察大鼠正畸牙周组织改建过程中基质金属蛋白酶—3(MMP—3)和金属蛋白酶组织抑制因子—1(TIMP—1)表达的变化，探讨MMP—3及TIMP—1与正畸牙齿移动的关系。方法：在SD成年大鼠上颌右侧第一磨牙与上颌切牙之间安置正畸装置，建立大鼠磨牙移动实验模型。于牙齿移动1、3、5、7、14d后取材分别进行免疫组化染色、图像分析。结果：牙齿移动1d后，牙周组织细胞MMP—3表达增强，5d后MMP—3表达达到高峰，此时破骨细胞脑浆亦呈强阳性表达。以后MMP—3表达有所下降，但仍高于对照组。而TIMP—1于牙齿移动3d后表达开始增强，7d后显著表达。结论：MMP—3及TIMP—1参与了正畸牙周组织改建过程，MMP—3在破骨细胞性骨吸收中起着重要作用。

The Expression of MMP-3 and TIMP-1 During Orthodontic Periodontium Remodeling in Rats

Objective:To observe the change of matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinase-1(TIMP-1) expression during experimental tooth movement in rats,and to learn about the relationship between MMP-3, TIMP-1 and orthodontic periodontal tissue remodeling.Methods:Orthodontic appliance was placed between the maxillary right first molar and maxillary central incisors of adult SD rat.Established rat molar movement model. Immunohistochemistry and image analyses were performed at 1?3?5?7?14 days after orthodontic force application.Results:The expression of MMP-3 and TIMP-1 were weak immunopostive in normal periodotium.At 1d after tooth movement, the immunoreactivities of MMP-3 were increased, at 5d , reached the maximum.Osteoclasts were also observed strong positive.The expression of TIMP-1 was increased at 3 days.At 7 days,a marked increase in TIMP-1-positive firblasts and osteoblasts was found not only on the pressure side but also on the tension side of the periodontium.Conclusions:MMP-3 and TIMP-1 participate in orthodontic periodontal tissue remodeling during tooth movement. MMP-3 may play an important role in osteoclastic bone resorption.