| 东莞市艾滋病病毒1型的分子流行病学研究 |
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| 引用本文： | 张巧利,夏宪照,冯铁建,王水能,王晓霞,杨挺立,陈婉雯. 东莞市艾滋病病毒1型的分子流行病学研究[J]. 热带医学杂志, 2006, 6(9): 994-996 |
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| 作者姓名： | 张巧利 夏宪照 冯铁建 王水能 王晓霞 杨挺立 陈婉雯 |
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| 作者单位： | 东莞市疾病预防控制中心,东莞,523006;深圳市疾病预防控制中心,深圳,518020 |
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| 摘 要： | 目的了解东莞市不同人群中艾滋病病毒(HIV)各亚型毒株的流行情况和传播规律。方法采集东莞市经蛋白印迹法确认的24名HIV感染者的外周抗凝血,分离单核细胞、提取核酸后用套式聚合酶链反应扩增HIV-1膜蛋白(env)基因C2-V3区的核酸片段,进行序列测定,鉴定病毒亚型。用威斯康星GCG软件进行共享序列、基因离散率的计算和毒株的聚类分析。结果在24份阳性标本中,有13份标本PCR扩增阳性,亚型分析结果表明东莞市存在HIV-1B和CRF01-AE两种亚型。结论HIV-1B和CRF01-AE可能是东莞市艾滋病病毒流行的主要亚型。
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| 关 键 词： | 人类免疫缺陷病毒 基因亚型 分子流行病学 |
| 文章编号： | 1672-3619（2006）09-0994-03 |
| 收稿时间： | 2006-03-10 |
| 修稿时间： | 2006-05-18 |
Molecular Epidemiology of HIV-1 of Dongguan City |
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| ZHANG Qiao-li,XIA Xian-zhao,FENG Tie-jian,WANG Shui-neng,WANG Xiao-xia,YANG Ting-li,CHEN Wan-wen. Molecular Epidemiology of HIV-1 of Dongguan City[J]. Journal Of Tropical Medicine, 2006, 6(9): 994-996 |
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| Authors: | ZHANG Qiao-li XIA Xian-zhao FENG Tie-jian WANG Shui-neng WANG Xiao-xia YANG Ting-li CHEN Wan-wen |
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| Abstract: | Objective To study the prevalence status and transmission patterns of different subtypes of HIV-1 in Dongguan city. Methods Peripheral mononucleated cells were prepared from 24 HIV carriers confirmed by Western Blotting. The provirus DNA was extracted and amplified by nested-PCR, after which the HIV-1 membrane protein gene (env) C2-V3 region was sequenced and its subtype was analyzed. Analyses of the consensus sequence, gene divergence and clustering of virus strains were conducted with Winsconson GCG software. Results Among 24 anti-HIV-1 antibody positive samples, 13 HIV-1 env gene fragments were amplified and analyzed. Subtypes B and E of HIV-1 virus strains were found in Dongguan city. Conclusion Subtype B and E of HIV-1 are probably main epidemiological strains in Dongguan. |
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| Keywords: | human immunodeficency virus gene subtype molecular epidemiology |
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