Diversity of Fusarium species causing invasive and disseminated infections |
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| Affiliation: | 1. Programa de Pós-graduação em Medicina: Ciências Médicas, Universidade Federal do Rio Grande do Sul, Brazil;2. Experimental Research Center, Hospital de Clínicas de Porto Alegre, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil;3. Microbiology Unit, Hospital de Clínicas de Porto Alegre, Brazil;4. Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Brazil;1. Global Action Fund for Fungal Infections (GAFFI), Geneva, Switzerland;2. Univ Rennes, CHU, Inserm, Irset (Institut de recherche en santé, environnement et travail) – UMR_S 1085, F-35000 Rennes, France;3. Société Française de Mycologie Médicale (SFMM), Paris, France;4. Manchester Fungal Infection Group, The University of Manchester, Manchester, United Kingdom;1. Department of Microbiology, Government Medical College Hospital, Sector 32-B, Chandigarh, India;2. Department of Dermatology, Government Medical College Hospital, Sector 32-B, Chandigarh, India;3. Department of Pathology, Government Medical College Hospital, Sector 32-B, Chandigarh, India;1. School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, China;2. Molecular Biotechnology Platform, Public Center of Experimental Technology, Southwest Medical University, Luzhou, 646000, China;3. Laboratory of Molecular Pharmacology, Department of Pharmacology, School of Pharmacy, Southwest Medical University, Luzhou, 646000, China;1. Microscopy Laboratory, Regional University of Cariri (URCA), Crato, CE, Brazil;2. Biology and Toxicology Laboratory, Regional University of Cariri (URCA), Crato, CE, Brazil;3. Microbiology and Molecular Biology Laboratory – LMBM, Regional University of Cariri – URCA, Crato, CE, Brazil |
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| Abstract: | IntroductionInvasive fusariosis (IF) is considered an emerging fungal disease and an important problem worldwide that increasingly affects immunocompromised individuals. There is currently concern about establishing the genetic diversity and phylogenetic relationship of the species Fusarium causing invasive fusariosis.Materials and MethodsThe aim of this study was to characterize the molecular profile and morphological characteristics of Fusarium species isolated from 21 patients with invasive fusariosis. Multilocus sequence typing was performed for molecular identification of the following genes: the second largest subunit of the RNA polymerase gene (RPB2) and elongation factor 1 alpha (EF-1α). The morphological features of different species were carefully described and revised by experienced mycologists.ResultsMorphological and molecular analyses revealed that the F. solani species complex (FSSC) and F. oxysporum species complex (FOSC) were the most common species isolated from patients with invasive fusariosis; FSSC-2 h (5), FSSC-1 (2) and FOSC-183 (2) were the most frequent haplotypes. The macroscopic characterization revealed great variation in the tonalities of the FSSC colonies and particularities amongst the species in relation to the macroconidia structures, while the FOSC was more homogeneous and presented shades from white to lilac.ConclusionsOur study characterized the diversity, haplotypes, and morphological aspects of Fusarium species and the haplotypes prevalent in patients with invasive fusariosis. FSSC and FSSC-2 h were the predominant species and haplotype, respectively. Although we have described interesting morphological aspects in Fusarium species, particularly haplotypes, their identification cannot rely on phenotypical aspects. Molecular biology techniques are necessary and should be introduced for routine use in mycology laboratories. |
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